Departamento de Química, División de Ciencias Naturales y Exactas, Universidad de Guanajuato, Noria Alta S/N, Colonia Noria Alta, Guanajuato, Guanajuato 36050, Mexico.
Molecules. 2017 Nov 7;22(11):1917. doi: 10.3390/molecules22111917.
(YL) is a "non-conventional" yeast that is capable of producing important metabolites. One of the most important products that is secreted by this microorganism is lipase, a ubiquitous enzyme that has considerable industrial potential and can be used as a biocatalyst in the pharmaceutical, food, and environmental industries. In this work, lipase (YLL) was immobilized on Lewatit and Amberlite beads and is used in the enzymatic ring-opening polymerization (ROP) of cyclic esters in the presence of different organic solvents. YLL immobilized on Amberlite XAD7HP had the higher protein adsorption (96%) and a lipolytic activity of 35 U/g. Lewatit VPOC K2629 has the higher lipolytic activity (805 U/g) and 92% of protein adsorption. The highest molecular weight (Mn 10,685 Da) was achieved at 90 °C using YLL that was immobilized on Lewatit 1026 with decane as solvent after 60 h and 100% of monomer conversion.
(YL) 是一种“非常规”酵母，能够产生重要的代谢物。该微生物分泌的最重要的产物之一是脂肪酶，脂肪酶是一种普遍存在的酶，具有相当大的工业潜力，可用作制药、食品和环境行业的生物催化剂。在这项工作中，脂肪酶 (YLL) 被固定在 Lewatit 和 Amberlite 珠上，并在不同有机溶剂的存在下用于环状酯的酶促开环聚合 (ROP)。固定在 Amberlite XAD7HP 上的 YLL 具有较高的蛋白质吸附率（96%）和 35 U/g 的脂肪酶活性。Lewatit VPOC K2629 具有较高的脂肪酶活性（805 U/g）和 92%的蛋白质吸附率。在 90°C 下，使用 Lewatit 1026 作为溶剂，在 60 小时后实现了最高分子量（Mn 10685 Da）和 100%的单体转化率。
