Key Laboratory for Molecular Enzymology and Engineering of Ministry of Education, College of Life Science, Jilin University, Changchun, 130012, China.
Appl Biochem Biotechnol. 2013 May;170(2):399-405. doi: 10.1007/s12010-013-0152-z. Epub 2013 Mar 28.
The paper explored the catalytic activity of a cell debris self-immobilized thermophilic lipase for polyester synthesis, using the ring-opening polymerization of ε-caprolactone as model. Effects of biocatalyst concentration, temperature, and reaction medium on monomer conversion and product molecular weight were systematically evaluated. The biocatalyst displayed high catalytic activity at high temperatures (70-90 °C), with 100 % monomer conversion. High monomer conversion values (>90 %) were achieved in both hydrophobic and hydrophilic solvents, and also in solvent-free system, with the exception of dichloromethane. Poly(ε-caprolactone) was obtained in 100 % monomer conversion, with a number-average molecular weight of 1,680 g/mol and a polydispersity index of 1.35 in cyclohexane at 70 °C for 72 h. Furthermore, the biocatalyst exhibited excellent operational stability, with monomer conversion values exceeding 90 % over the course of 15 batch reactions.
本文探讨了细胞碎片自固定嗜热脂肪酶在聚酯合成中的催化活性，以ε-己内酯的开环聚合反应作为模型。系统评估了生物催化剂浓度、温度和反应介质对单体转化率和产物分子量的影响。该生物催化剂在高温（70-90°C）下表现出高催化活性，单体转化率达到 100%。在疏水性和亲水性溶剂中，以及在无溶剂体系中，除二氯甲烷外，都能达到高单体转化率值（>90%）。在 70°C 下反应 72 小时，在环己烷中，ε-己内酯的单体转化率为 100%，得到的聚己内酯的数均分子量为 1680g/mol，多分散指数为 1.35。此外，该生物催化剂表现出优异的操作稳定性，在 15 批连续反应中，单体转化率超过 90%。
