Sager R, Kovac P E
Somatic Cell Genet. 1979 Jul;5(4):491-502. doi: 10.1007/BF01538883.
Hybrid cells produced by the fusion of pairs of cells, one a tumorigenic derivative of CHEF/16 and the other a nontumorigenic derivative of CHEF/18, give rise to clones which are largely tetraploid, but rare reduced hybrids with chromosome counts in the diploid range have been recovered from tumors of hybrid origin. This paper describes the recovery in cell culture of reduced hybrids in the diploid range by selection with 5-bromodeoxyuridine (BrdU) or methylcellulose as well as by growth in culture of cells from excised tumors. All selected subclones were tumorigenic and resistant to BrdU, but they segregated for resistance to 6-thioguanine. Unselected subclones were tetraploid, nontumorigenic, and sensitive to both drugs. These data show that chromosome reassortment as well as extensive chromosome reduction both occur in a small fraction of the population during growth of each hybrid clone.
由CHEF/16的致瘤性衍生物细胞与CHEF/18的非致瘤性衍生物细胞两两融合产生的杂交细胞，可形成大部分为四倍体的克隆，但从杂交起源的肿瘤中已获得了罕见的染色体数在二倍体范围内的减数杂交细胞。本文描述了通过用5-溴脱氧尿苷（BrdU）或甲基纤维素进行选择以及通过切除肿瘤的细胞在培养中生长，在细胞培养中获得二倍体范围内的减数杂交细胞。所有选择的亚克隆均具有致瘤性且对BrdU有抗性，但它们对6-硫鸟嘌呤的抗性出现了分离。未选择的亚克隆是四倍体、非致瘤性的，并且对两种药物均敏感。这些数据表明，在每个杂交克隆生长过程中，一小部分群体中会发生染色体重排以及广泛的染色体减少。
