Bouck N, di Mayorca G
Mol Cell Biol. 1982 Feb;2(2):97-105. doi: 10.1128/mcb.2.2.97-105.1982.
Treatment of BHK cells with mutagenic carcinogens induced neoplastic transformation in a single step. This transformation displayed the characteristics expected for a recessive mutation. Increasing doses of carcinogens induced transformants with kinetics similar to the kinetics with which they induced 6-thioguanine-resistant or ouabain-resistant mutants in the same population of cells. Transformants with temperature-restricted phenotypes were easily induced by carcinogens which cause mutations by base changes, but when ICR frameshift mutagens were used, the proportion of temperature-limited transformants was inversely related to the frequency with which a particular mutagen induced frameshift mutations. In hybrids between pseudodiploid isogenic strains of normal and transformed BHK cells, transformation was expressed as a dominant trait when the transformed parent was induced by a papovavirus, but was suppressed as a recessive trait when the transformed parent arose spontaneously or was chemically induced. Segregation of transformation was observed upon growth of suppressed normal hybrids, and the transformed phenotype which was reexpressed was in most cases characteristics of the original transformed parent.
用诱变致癌物处理BHK细胞可一步诱导肿瘤转化。这种转化表现出隐性突变预期的特征。致癌剂剂量增加诱导转化体的动力学，与它们在同一细胞群体中诱导6-硫鸟嘌呤抗性或哇巴因抗性突变体的动力学相似。碱基改变引起突变的致癌物很容易诱导出具有温度限制表型的转化体，但是当使用ICR移码诱变剂时，温度限制转化体的比例与特定诱变剂诱导移码突变的频率呈负相关。在正常BHK细胞和转化BHK细胞的假二倍体同基因菌株之间的杂种中，当转化亲本由乳头多瘤病毒诱导时，转化表现为显性性状，但当转化亲本自发产生或化学诱导产生时，转化表现为隐性性状被抑制。在被抑制的正常杂种生长时观察到转化的分离，重新表达的转化表型在大多数情况下是原始转化亲本的特征。
