Mohd Azhar Siti Hajar, Abdulla Rahmath, Jambo Siti Azmah, Marbawi Hartinie, Gansau Jualang Azlan, Mohd Faik Ainol Azifa, Rodrigues Kenneth Francis
Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
Energy Research Unit, Universiti Malaysia Sabah, Jalan UMS, 88400 Kota Kinabalu, Sabah, Malaysia.
Biochem Biophys Rep. 2017 Mar 6;10:52-61. doi: 10.1016/j.bbrep.2017.03.003. eCollection 2017 Jul.
Bioethanol has been identified as the mostly used biofuel worldwide since it significantly contributes to the reduction of crude oil consumption and environmental pollution. It can be produced from various types of feedstocks such as sucrose, starch, lignocellulosic and algal biomass through fermentation process by microorganisms. Compared to other types of microoganisms, yeasts especially is the common microbes employed in ethanol production due to its high ethanol productivity, high ethanol tolerance and ability of fermenting wide range of sugars. However, there are some challenges in yeast fermentation which inhibit ethanol production such as high temperature, high ethanol concentration and the ability to ferment pentose sugars. Various types of yeast strains have been used in fermentation for ethanol production including hybrid, recombinant and wild-type yeasts. Yeasts can directly ferment simple sugars into ethanol while other type of feedstocks must be converted to fermentable sugars before it can be fermented to ethanol. The common processes involves in ethanol production are pretreatment, hydrolysis and fermentation. Production of bioethanol during fermentation depends on several factors such as temperature, sugar concentration, pH, fermentation time, agitation rate, and inoculum size. The efficiency and productivity of ethanol can be enhanced by immobilizing the yeast cells. This review highlights the different types of yeast strains, fermentation process, factors affecting bioethanol production and immobilization of yeasts for better bioethanol production.
生物乙醇已被确定为全球使用最广泛的生物燃料,因为它对减少原油消耗和环境污染有显著贡献。它可以通过微生物发酵过程,由各种原料如蔗糖、淀粉、木质纤维素和藻类生物质生产。与其他类型的微生物相比,酵母尤其是乙醇生产中常用的微生物,因为它具有高乙醇生产率、高乙醇耐受性以及发酵多种糖类的能力。然而,酵母发酵存在一些抑制乙醇生产的挑战,如高温、高乙醇浓度以及发酵戊糖的能力。各种类型的酵母菌株已用于乙醇生产的发酵,包括杂交酵母、重组酵母和野生型酵母。酵母可以直接将单糖发酵成乙醇,而其他类型的原料必须先转化为可发酵糖才能发酵成乙醇。乙醇生产的常见过程包括预处理、水解和发酵。发酵过程中生物乙醇的产量取决于几个因素,如温度、糖浓度、pH值、发酵时间、搅拌速度和接种量。固定化酵母细胞可以提高乙醇的效率和生产率。本综述重点介绍了不同类型的酵母菌株、发酵过程、影响生物乙醇生产的因素以及酵母固定化以实现更好的生物乙醇生产。