Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Kocaeli, Turkey.
Department of Environmental Engineering, Gebze Institute of Technology, 41400 Gebze, Kocaeli, Turkey.
Bioresour Technol. 2014 Apr;158:286-93. doi: 10.1016/j.biortech.2014.02.022. Epub 2014 Feb 15.
In this research, Scheffersomyces stipitis and Saccharomyces cerevisiae in immobilized and suspended state were used to convert pentose and hexose sugars to ethanol. In batch and continuous systems, S. stipitis and S. cerevisiae co-culture performance was better than S. cerevisiae. Continuous ethanol production was performed in packed bed immobilized cell reactor (ICR). In ICR, S. stipitis cells were found to be more sensitive to oxygen concentration and other possible mass transfer limitations as compared to S. cerevisiae. Use of co-immobilized S. stipitis and S. cerevisiae resulted in maximum xylose consumption (73.92%) and 41.68 g/L day ethanol was produced at HRT (hydraulic retention time) of 6h with wheat straw hydrolysate. At HRT of 0.75 h, the highest amount of ethanol with the values of 356.21 and 235.43 g/L day was produced when synthetic medium and wheat straw hydrolysate were used as feeding medium in ICR, respectively.
在这项研究中，使用固定化和悬浮状态的毕赤酵母和酿酒酵母将戊糖和己糖转化为乙醇。在分批和连续系统中，毕赤酵母和酿酒酵母共培养的性能优于酿酒酵母。连续乙醇生产在填充床固定化细胞反应器（ICR）中进行。在 ICR 中，与酿酒酵母相比，毕赤酵母细胞对氧气浓度和其他可能的传质限制更为敏感。使用共固定化的毕赤酵母和酿酒酵母可实现最大木糖消耗（73.92%），在水力停留时间（HRT）为 6 小时的情况下，利用麦草水解物可生产 41.68 g/L 天的乙醇。在 HRT 为 0.75 小时时，当使用合成培养基和麦草水解物作为 ICR 中的进料介质时，分别产生了最高的乙醇量，其值分别为 356.21 和 235.43 g/L 天。
