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GATAAG；一个顺式调控区域，可结合一种对珠蛋白和非珠蛋白基因表达起作用的红系特异性核因子。

GATAAG; a cis-control region binding an erythroid-specific nuclear factor with a role in globin and non-globin gene expression.

作者信息

Plumb M, Frampton J, Wainwright H, Walker M, Macleod K, Goodwin G, Harrison P

机构信息

Beatson Institute for Cancer Research, Glasgow, UK.

出版信息

Nucleic Acids Res. 1989 Jan 11;17(1):73-92. doi: 10.1093/nar/17.1.73.

DOI:10.1093/nar/17.1.73
PMID:2911489
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC331536/
Abstract

An erythroid-specific nuclear protein factor binds to a sequence motif (GATAAG) which is present in the promoter region of the mouse alpha and beta major globin genes, and in the erythroid-specific promoter of the human porphobilinogen deaminase (PBG-D) gene. The protein activity is conserved across species, being found in mouse erythroleukaemia (MEL) cells, chicken erythrocytes, the human erythroid K562 and KMOE cell lines, but not in a variety of non-erythroid mouse tissues or in HeLa cells. Functional analysis of this element in the alpha globin gene promoter by stable transfection experiments show that the GATAAG motif resides in a 68 bp sequence which has a stimulatory effect on transcription in mouse erythroleukaemia but not fibroblast cells. The GATAAG motif is conserved in the promoters and 3' enhancers of a variety of globin and non-globin genes implying that it is a cis-element involved in the tissue-specific up-regulation of several genes that are co-expressed during erythroid cell differentiation.

摘要

一种红系特异性核蛋白因子与一个序列基序（GATAAG）结合，该基序存在于小鼠α和β主要珠蛋白基因的启动子区域以及人胆色素原脱氨酶（PBG-D）基因的红系特异性启动子中。这种蛋白质活性在物种间保守，在小鼠红白血病（MEL）细胞、鸡红细胞、人红系K562和KMOE细胞系中存在，但在多种非红系小鼠组织或HeLa细胞中不存在。通过稳定转染实验对α珠蛋白基因启动子中该元件进行功能分析表明，GATAAG基序位于一个68 bp的序列中，该序列对小鼠红白血病细胞而非成纤维细胞中的转录有刺激作用。GATAAG基序在多种珠蛋白和非珠蛋白基因的启动子和3'增强子中保守，这意味着它是一个顺式元件，参与了红系细胞分化过程中几个共表达基因的组织特异性上调。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/2298323a0a04/nar00210-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/cb5737d81ea7/nar00210-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/50706d921cd8/nar00210-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/14e339ecad3d/nar00210-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/f4721bfe7cf6/nar00210-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/7045aebc6bfe/nar00210-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/2298323a0a04/nar00210-0102-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/cb5737d81ea7/nar00210-0093-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/50706d921cd8/nar00210-0094-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/14e339ecad3d/nar00210-0097-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/f4721bfe7cf6/nar00210-0098-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/7045aebc6bfe/nar00210-0100-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/86fc/331536/2298323a0a04/nar00210-0102-a.jpg

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