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赋予鸡成年β-珠蛋白基因5'端核酸酶超敏感性的特定因子。

Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.

作者信息

Emerson B M, Felsenfeld G

出版信息

Proc Natl Acad Sci U S A. 1984 Jan;81(1):95-9. doi: 10.1073/pnas.81.1.95.

DOI:10.1073/pnas.81.1.95
PMID:6320177
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC344617/
Abstract

Chromatin in the 5' flanking region of the transcriptionally active chicken adult beta(beta A) globin gene is hypersensitive to nuclease digestion. When plasmids carrying this gene are combined with histones in the presence of extracts from 9-day-old chicken erythrocyte nuclei, the resultant complex displays hypersensitive behavior in the same region. No such behavior is observed with extracts from cells in which the beta A-globin gene is not expressed. Partially purified preparations of the factor conferring hypersensitivity bind preferentially to DNA fragments containing the hypersensitive region.

摘要

转录活跃的鸡成年β（βA）珠蛋白基因5'侧翼区域的染色质对核酸酶消化高度敏感。当携带该基因的质粒在9日龄鸡红细胞核提取物存在的情况下与组蛋白结合时，形成的复合物在同一区域表现出高度敏感行为。在βA珠蛋白基因不表达的细胞提取物中未观察到这种行为。赋予高度敏感性的因子的部分纯化制剂优先结合含有高度敏感区域的DNA片段。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/9a41f48e6951/pnas00602-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/f280a1f64d3f/pnas00602-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/b6ed728c9c20/pnas00602-0111-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/fd76550e0f75/pnas00602-0111-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/b28d19a59c79/pnas00602-0111-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/9a41f48e6951/pnas00602-0112-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/f280a1f64d3f/pnas00602-0111-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/b6ed728c9c20/pnas00602-0111-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/fd76550e0f75/pnas00602-0111-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/b28d19a59c79/pnas00602-0111-d.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2924/344617/9a41f48e6951/pnas00602-0112-a.jpg

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Specific factor conferring nuclease hypersensitivity at the 5' end of the chicken adult beta-globin gene.赋予鸡成年β-珠蛋白基因5'端核酸酶超敏感性的特定因子。
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9
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10
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