(Ruiz & Pavón, 1799) B chromosome shares repetitive DNA sequences with A chromosomes of different (Linnaeus, 1753) species.

Species of (Linnaeus, 1753) have shown large diversity in the accumulation and distribution of repetitive DNA families, and B chromosomes have been described in seven species. Some types of repetitive DNA were identified in A and B chromosomes in species of this plant group, such as AT-rich SSR, 35S and 5S rDNA, C-Giemsa and C-CMA/DAPI bands and retrotransposons. To increase our understanding of the relationships of A and B chromosomes, the B of Ruiz & Pavón, 1799 was microdissected, amplified and hybridized against chromosomes of this species, and in six other species of this genus. FISH signals were observed in whole the B of , including stretches of A chromosomes, as well as in some A chromosomes of all tested species. A strong FISH signal was seen adjacent to the 5S rDNA in the proximal region of pair 8 of all species and, due to this, we have searched for 5S rDNA fragments in the microdissected B chromosome. PCR and sequencing data evidenced 5S rDNA deletion along evolutionary pathways of the B of . Although A and B chromosomes displayed redundancy in the repetitive DNA families in different species, the B of seemed to differ from those Bs of other species by the loss of rDNA fractions. A possible origin of Bs in was discussed.