Matsuzaki Shigenobu, Uchiyama Jumpei, Takemura-Uchiyama Iyo, Ujihara Takako, Daibata Masanori
Department of Microbiology and Infection, Kochi Medical School, Kochi University, Kohasu, Oko-cho, Nankoku, Kochi, 783-8505, Japan.
School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa, 252-0206, Japan.
Methods Mol Biol. 2018;1693:3-10. doi: 10.1007/978-1-4939-7395-8_1.
One of the most important factors for successful bacteriophage therapy is, undoubtedly, the isolation of excellent therapeutic candidate bacteriophages. There are only a few reports about active bacteriophages in the fastidious bacteria Helicobacter pylori. In this chapter, we describe a method for isolating and purifying KHP30-like bacteriophages in H. pylori, which have lytic and pseudolysogenic life cycles.
毫无疑问，成功进行噬菌体治疗的最重要因素之一是分离出优秀的治疗候选噬菌体。关于苛养菌幽门螺杆菌中活性噬菌体的报道很少。在本章中，我们描述了一种在幽门螺杆菌中分离和纯化KHP30样噬菌体的方法，这些噬菌体具有裂解性和假溶原性生命周期。
