Agboluaje Maryam, Sauvageau Dominic
Chemical and Materials Engineering, University of Alberta, 12th floor Donadeo ICE Building, 9211 116 St NW, Edmonton, AB, Canada, T5M 0L5.
Methods Mol Biol. 2018;1693:173-193. doi: 10.1007/978-1-4939-7395-8_15.
The optimal conditions for the production of virulent bacteriophages in bioreactors can vary greatly depending on the host-bacteriophage system used. We present a general method for the production of virulent bacteriophages in bioreactors that can be adapted to many host-bacteriophage systems and various operating conditions (reactor volume, medium composition, temperature, etc.). The procedures detail how to establish optimal initial infection conditions (infection load and initial multiplicity of infection (MOI)), prepare the host pre-culture and bioreactor, operate the bioreactor, and harvest the bacteriophage product. Batch operation is detailed but a short discussion addresses other modes of operation, namely two-stage continuous bioreactors and two-stage cycling bioreactors.
在生物反应器中生产烈性噬菌体的最佳条件会因所使用的宿主-噬菌体系统而有很大差异。我们提出了一种在生物反应器中生产烈性噬菌体的通用方法，该方法可适用于许多宿主-噬菌体系统以及各种操作条件（反应器体积、培养基组成、温度等）。这些步骤详细说明了如何建立最佳初始感染条件（感染量和初始感染复数（MOI））、制备宿主预培养物和生物反应器、操作生物反应器以及收获噬菌体产物。文中详细介绍了分批操作，但也简要讨论了其他操作模式，即两级连续生物反应器和两级循环生物反应器。
