a Department of Applied Biology, School of Applied Natural Sciences , Adama Science and Technology University , Adama , Ethiopia.
b Department of Animal Biotechnology , Konkuk University , Seoul , South Korea.
Anim Biotechnol. 2018;29(4):293-300. doi: 10.1080/10495398.2017.1383915. Epub 2017 Nov 9.
Promoters are, generally, located immediately upstream of a transcription start site (TSS) and have a variety of regulatory motifs, such as transcription factors (TFs) and CpG islands (CGIs), that participate in the regulation of gene expression. Here analysis of the promoter region for pig vomeronasal receptor type 1 (V1R) was described. In the analysis, TSSs for pig V1R genes was first identified and five motifs (MV1, MV2, MV3, MV4, and MV5) were found that are shared by at least 50% of the pig V1R promoter input sequences from both strands. Among the five motifs, MV2 was identified as a common promoter motif shared by all (100%) pig V1R promoters. For further analysis, to better characterize and get deeper biological insight associated with MV2, TOMTOM web application was used. MV2 was compared to the known motif databases (such as JASPAR) to see if they are similar to a known regulatory motif (transcription factor). Hence, it was revealed that MV2 serves as the binding site mainly for the BetaBetaAlpha-zinc finger (BTB-ZF) transcription factor gene family to regulate expression of pig V1R genes. Moreover, it was shown that pig V1R promoters are CpG poor, suggesting that their gene expression regulation pattern is in tissue specific manner.
启动子通常位于转录起始位点（TSS）的上游，具有多种调节元件，如转录因子（TFs）和 CpG 岛（CGIs），参与基因表达的调控。本文描述了猪嗅觉受体 1 型（V1R）启动子区域的分析。在分析中，首先确定了猪 V1R 基因的 TSS，并发现了至少 50%的猪 V1R 启动子输入序列共享的五个基序（MV1、MV2、MV3、MV4 和 MV5），这些序列来自两条链。在这五个基序中，MV2 被鉴定为所有（100%）猪 V1R 启动子共有的常见启动子基序。为了进一步分析，更好地描述和深入了解与 MV2 相关的生物学信息，使用了 TOMTOM 网络应用程序。将 MV2 与已知的基序数据库（如 JASPAR）进行比较，以查看它们是否与已知的调节基序（转录因子）相似。因此，揭示了 MV2 作为 BetaBetaAlpha-zinc finger（BTB-ZF）转录因子基因家族的结合位点，调节猪 V1R 基因的表达。此外，还表明猪 V1R 启动子富含 CpG，表明它们的基因表达调控模式是组织特异性的。
