College of Food Science and Technology, Huazhong Agricultural University, Wuhan, PR China.
Food Chem. 2018 Apr 1;244:232-237. doi: 10.1016/j.foodchem.2017.10.071. Epub 2017 Oct 12.
Pectin oligosaccharide (POS) fractions were obtained by controlled chemical degradation of citrus peel pectin. By adjusting trifluoroacetic acid (TFA) concentration, three oligosaccharides were obtained, of molecular weight (Mw) range 3000-4000 Da, 2000-3000 Da and lower than 2000 Da. Varying hydrogen peroxide (HO) concentration and reaction time produced oligosaccharides of 3000-4000 Da and 2000-3000 Da. The relative proportions of acidic monosaccharide increased from 68.58% to 89.93% (TFA) and from 63.74% to 83.26% (HO) as the reaction conditions intensified. Prebiotic activity scores were used to quantify POS ability to promote selective growth of specific probiotics. Sample POS from HO degradation showed the highest prebiotic potential with prebiotic activity score 0.41 for Lactobacillus paracasei LPC-37 and 0.92 for Bifidobacterium bifidum ATCC 29521.
果胶低聚糖（POS）级分通过控制柑橘皮果胶的化学降解获得。通过调整三氟乙酸（TFA）浓度，可以得到分子量（Mw）范围在 3000-4000Da、2000-3000Da 和低于 2000Da 的三种低聚糖。改变过氧化氢（HO）浓度和反应时间可以得到 3000-4000Da 和 2000-3000Da 的低聚糖。随着反应条件的加强，酸性单糖的相对比例从 68.58%增加到 89.93%（TFA）和从 63.74%增加到 83.26%（HO）。使用益生元活性评分来量化 POS 促进特定益生菌选择性生长的能力。来自 HO 降解的样品 POS 显示出最高的益生元潜力，对副干酪乳杆菌 LPC-37 的益生元活性评分为 0.41，对双歧杆菌 bifidum ATCC 29521 的益生元活性评分为 0.92。
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