Department of Food Science & Technology, College of Agricultural and Environmental Sciences, The University of Georgia, 100 Cedar Street, Athens, GA 30602, United States.
Department of Pharmaceutical & Biomedical Sciences, College of Pharmacy, The University of Georgia, 250 W. Green Street, Athens, GA 30602, United States.
Food Chem. 2018 Apr 1;244:359-363. doi: 10.1016/j.foodchem.2017.10.035. Epub 2017 Oct 10.
In vitro assays are widely used to analyze the antioxidant potential of compounds, but they cannot accurately predict antioxidant behavior in living systems. Cell-based assays, like the cellular antioxidant activity (CAA) assay, are gaining importance as they provide a biological perspective. When the CAA assay was employed to study phenolic antioxidants using hepatocarcinoma (HepG2) cells, quercetin showed antioxidant activity in HepG2 cells; 25 and 250μM quercetin reduced fluorescence by 17.1±0.9% and 58.6±2.4%, respectively. (+)-Catechin, a phenolic antioxidant present in many foods, bestowed virtually no CAA in HepG2 cells. When Caco-2 cells were employed, more robust antioxidant activity was observed; 50μM (+)-catechin and quercetin reduced fluorescence by 54.1±1.4% and 63.6±0.9%, respectively. Based on these results, likely due to differences in active membrane transport between the cell types, the Caco-2-based CAA assay appears to be a more appropriate method for the study of certain dietary phenolics.
体外测定法被广泛用于分析化合物的抗氧化潜力，但它们无法准确预测化合物在活体内的抗氧化行为。基于细胞的测定法，如细胞抗氧化活性（CAA）测定法，越来越受到重视，因为它们提供了一种生物学视角。当 CAA 测定法被用于使用肝癌（HepG2）细胞研究酚类抗氧化剂时，槲皮素在 HepG2 细胞中表现出抗氧化活性；25 和 250μM 槲皮素分别使荧光降低了 17.1±0.9%和 58.6±2.4%。（+）-儿茶素是许多食物中存在的一种酚类抗氧化剂，几乎没有赋予 HepG2 细胞 CAA。当使用 Caco-2 细胞时，观察到更强的抗氧化活性；50μM（+）-儿茶素和槲皮素使荧光分别降低了 54.1±1.4%和 63.6±0.9%。基于这些结果，可能由于细胞类型之间主动膜转运的差异，基于 Caco-2 的 CAA 测定法似乎更适合某些膳食酚类物质的研究。
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