Wang Jianye, Ling Jueyi, Wang Zhixian, Huang Yu, Zhu Jianzhong, Zhu Guoqiang
College of Veterinary Medicine, Yangzhou University, 48 Wenhui East Road, 225009, Yangzhou, Jiangsu Province, People's Republic of China.
Jiangsu Co-Innovation Center for Important Animal Infectious Diseases and Zoonoses, Yangzhou, 225009, China.
BMC Vet Res. 2017 Nov 9;13(1):327. doi: 10.1186/s12917-017-1238-6.
Muscovy duck parvovirus (MDPV) and Goose parvovirus (GPV) are important etiological agents for Muscovy duck parvoviral disease and Derzsy's disease, respectively; both of which can cause substantial economic losses in waterfowl industry. In contrast to GPV, the complete genomic sequence data of MDPV isolates are still limited and their phylogenetic relationships largely remain unknown. In this study, the entire genome of a pathogenic MDPV strain ZW, which was isolated from a deceased Muscovy duckling in 2006 in China, was cloned, sequenced, and compared with that of other classical MDPV and GPV strains.
The genome of strain ZW comprises of 5071 nucleotides; this genome was shorter than that of the pathogenic MDPV strain YY (5075 nt). All the four deleted nucleotides produced in strain ZW are located at the base-pairing positions in the palindromic stem of inverted terminal repeats (ITR) without influencing the formation of a hairpin structure. Recombination analysis revealed that strain ZW originated from genetic recombination between the classical MDPV and GPV strain. The YY strain of MDPV acts as the major parent, whereas the virulent strains YZ99-6 and B and the vaccine strain SYG61v of GPV act as the minor parents in varying degrees. Two recombination sites were detected in strain ZW, with the small recombination site surrounding the P9 promoter, and the large recombination site situated in the middle of the VP3 gene. The SYG61V strain is a vaccine strain used for preventing goose parvoviral disease. This strain was found to be solely involved in the recombination event detected in the P9 promoter region. Phylogenetic analyses between strain ZW and other classical strains of MDPV and GPV were performed. The results supported the in silico recombination analysis conclusion.
MDPV Strain ZW is a novel recombinant parvovirus, and the bulk of its genome originates from the classical MDPV strain. Two virulent strains and a vaccine strain of GPV were involved in the recombination process in varying degrees.
番鸭细小病毒（MDPV）和鹅细小病毒（GPV）分别是番鸭细小病毒病和鹅细小病毒病的重要病原体；这两种病毒都会给水禽养殖业造成重大经济损失。与GPV相比，MDPV分离株的完整基因组序列数据仍然有限，其系统发育关系在很大程度上仍不清楚。在本研究中，从2006年中国一只死亡番鸭雏鸭中分离出的致病性MDPV毒株ZW的全基因组被克隆、测序，并与其他经典MDPV和GPV毒株进行比较。
ZW毒株的基因组由5071个核苷酸组成；该基因组比致病性MDPV毒株YY（5075 nt）的基因组短。ZW毒株中产生的所有四个缺失核苷酸都位于反向末端重复序列（ITR）回文茎中的碱基配对位置，而不影响发夹结构的形成。重组分析表明，ZW毒株起源于经典MDPV和GPV毒株之间的基因重组。MDPV的YY毒株是主要亲本，而GPV的强毒株YZ99 - 6和B以及疫苗株SYG61v在不同程度上是次要亲本。在ZW毒株中检测到两个重组位点，小重组位点围绕P9启动子，大重组位点位于VP3基因中间。SYG61V毒株是用于预防鹅细小病毒病的疫苗株。发现该毒株仅参与了在P9启动子区域检测到的重组事件。对ZW毒株与其他经典MDPV和GPV毒株进行了系统发育分析。结果支持了计算机重组分析结论。
MDPV毒株ZW是一种新型重组细小病毒，其大部分基因组起源于经典MDPV毒株。两种GPV强毒株和一种疫苗株在不同程度上参与了重组过程。
