Rodríguez V, Rivoira M, Guizzardi S, Tolosa de Talamoni N
Laboratorio "Dr. Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Córdoba, Argentina.
Laboratorio "Dr. Cañas", Cátedra de Bioquímica y Biología Molecular, Facultad de Ciencias Médicas, INICSA (CONICET-Universidad Nacional de Córdoba), Córdoba, Argentina.
Arch Biochem Biophys. 2017 Dec 15;636:1-10. doi: 10.1016/j.abb.2017.11.002. Epub 2017 Nov 7.
This study tries to elucidate the mechanisms by which fructose rich diets (FRD) inhibit the rat intestinal Ca absorption, and determine if any or all underlying alterations are prevented by naringin (NAR). Male rats were divided into: 1) controls, 2) treated with FRD, 3) treated with FRD and NAR. The intestinal Ca absorption and proteins of the transcellular and paracellular Ca pathways were measured. Oxidative/nitrosative stress and inflammation parameters were evaluated. FRD rats showed inhibition of the intestinal Ca absorption and decrease in the protein expression of molecules of both Ca pathways, which were blocked by NAR. FRD rats showed an increase in the superoxide anion, a decrease in the glutathione and in the enzymatic activities of the antioxidant system, as well as an increase in the NO content and in the nitrotyrosine content of proteins. They also exhibited an increase in both IL-6 and nuclear NF-κB. All these changes were prevented by NAR. In conclusion, FRD inhibit both pathways of the intestinal Ca absorption due to the oxidative/nitrosative stress and inflammation. Since NAR prevents the oxidative/nitrosative stress and inflammation, it might be a drug to avoid alteration in the intestinal Ca absorption caused by FRD.
本研究试图阐明富含果糖饮食（FRD）抑制大鼠肠道钙吸收的机制，并确定柚皮苷（NAR）是否能预防任何或所有潜在的改变。雄性大鼠被分为：1）对照组，2）接受FRD处理组，3）接受FRD和NAR处理组。测量肠道钙吸收以及跨细胞和细胞旁钙途径的蛋白质。评估氧化/亚硝化应激和炎症参数。FRD大鼠表现出肠道钙吸收受到抑制，且两条钙途径分子的蛋白质表达均降低，而这些均被NAR阻断。FRD大鼠表现出超氧阴离子增加、谷胱甘肽减少以及抗氧化系统的酶活性降低，同时蛋白质的NO含量和硝基酪氨酸含量增加。它们还表现出IL-6和核NF-κB均增加。所有这些变化均被NAR预防。总之，FRD由于氧化/亚硝化应激和炎症而抑制肠道钙吸收的两条途径。由于NAR可预防氧化/亚硝化应激和炎症，它可能是一种避免FRD引起肠道钙吸收改变的药物。
