Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India.
Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India.
Peptides. 2018 Jan;99:1-7. doi: 10.1016/j.peptides.2017.11.004. Epub 2017 Nov 6.
Exploring bone rebuilding anabolic agents has been gaining much attention due to their potential therapeutic effects in treating several bone disorders including osteoporosis. Whey protein has been reported to affect bone health osteoanabolically, in terms of proliferation and differentiation of primary osteoblast cells. This study investigates whether whey derived anti-oxidative (AO) (P1- MHIRL, P2- YVEEL) and angiotensin converting enzyme inhibitory (ACE inhibitory) (P3- YLLF, P4-ALPMHIR, P5-IPA, P6- WLAHK) bioactive peptides affect the proliferation and differentiation of primary osteoblast cells isolated from rat calvaria. The proliferation and osteogenic activity of osteoblast cells in presence of these peptides were determined by MTT assay, DNA quantification study, Alkaline phosphatase activity (ALP) and ALP staining, Alizarin red activity and staining, and secretory osteocalcin measurement. The expression of osteogenesis-related genes (COLI-α, ALP, OCN and RUNX2) were determined by real-time quantitative PCR (RT-PCR) analysis over a period of 21days. The peptide treated osteoblasts showed a significant increase in viable cell density and proliferation in the order of P2>P6>P3 at optimised concentration. Furthermore, the osteoblastic differentiation markers in response to these peptides were found to be significantly up regulated in the order of P2>P6>P3 when compared to the controls. These results demonstrated that bioactive whey-derived AO and ACE inhibitory peptides can play a potential therapeutic role in osteoporosis by activating osteoblasts anabolically.
由于其在治疗多种骨骼疾病（包括骨质疏松症）方面的潜在治疗效果，探索骨重建合成代谢剂一直备受关注。乳清蛋白已被报道在骨合成代谢方面影响骨骼健康，影响原代成骨细胞的增殖和分化。本研究旨在探讨乳清来源的抗氧化（AO）（P1-MHIRL、P2-YVEEL）和血管紧张素转化酶抑制（ACE 抑制）（P3-YLLF、P4-ALPMHIR、P5-IPA、P6-WLAHK）生物活性肽是否影响从大鼠颅骨分离的原代成骨细胞的增殖和分化。通过 MTT 测定法、DNA 定量研究、碱性磷酸酶活性（ALP）和 ALP 染色、茜素红活性和染色以及分泌性骨钙素测量来确定这些肽存在时成骨细胞的增殖和成骨活性。在 21 天的时间内，通过实时定量 PCR（RT-PCR）分析确定与成骨相关的基因（COLI-α、ALP、OCN 和 RUNX2）的表达。在优化浓度下，肽处理的成骨细胞的活细胞密度和增殖呈 P2>P6>P3 的顺序显著增加。此外，与对照组相比，这些肽对成骨细胞分化标志物的反应被发现呈 P2>P6>P3 的顺序显著上调。这些结果表明，生物活性乳清来源的 AO 和 ACE 抑制肽通过激活成骨细胞在合成代谢上可以在骨质疏松症中发挥潜在的治疗作用。
