de Oliveira Ana P, Coppede Juliana S, Bertoni Bianca W, Crotti Antônio E M, França Suzelei C, Pereira Ana Maria S, Taleb-Contini Silvia H
Unidade de Biotecnologia, Universidade de Ribeirão Preto, Ribeirão Preto, SP, CEP 14096-900, Brazil.
Departamento de Química, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, CEP 14040-901, Brazil.
Chem Biodivers. 2018 Jan;15(1). doi: 10.1002/cbdv.201700421. Epub 2017 Dec 27.
Costus spiralis, a plant used in traditional Brazilian medicine for the treatment of complications in diabetes, was investigated. Assay of hexane, ethyl acetate, methanol, and aqueous fractions obtained by partition of a crude methanol extract of dried leaves of C. spiralis revealed that AGI activity was confined to the ethyl acetate fraction. Purification of this fraction yielded schaftoside and isoschaftoside. The AGI activities of the two flavones were lower than, but comparable with, that of the anti-diabetic drug acarbose. In contrast, the IC value of the ethyl acetate fraction was 1.95-, 2.34-, and 2.22-fold higher than those of acarbose, schaftoside, and isoschaftoside, respectively. The results demonstrate for the first time that schaftoside and isoschaftoside are responsible, in part, for the AGI activity of C. spiralis. Our study suggests that further investigations into C. spiralis may lead to the discovery of additional compounds with antihyperglycemic activity.
对巴西传统医学中用于治疗糖尿病并发症的植物螺旋闭鞘姜进行了研究。对螺旋闭鞘姜干燥叶片粗甲醇提取物进行分配得到的己烷、乙酸乙酯、甲醇和水相部分的分析表明，α-葡萄糖苷酶抑制（AGI）活性仅限于乙酸乙酯部分。对该部分进行纯化得到schaftoside和异schaftoside。这两种黄酮的AGI活性低于抗糖尿病药物阿卡波糖，但与之相当。相比之下，乙酸乙酯部分的IC值分别比阿卡波糖、schaftoside和异schaftoside高1.95倍、2.34倍和2.22倍。结果首次证明schaftoside和异schaftoside部分地导致了螺旋闭鞘姜的AGI活性。我们的研究表明，对螺旋闭鞘姜的进一步研究可能会发现具有降血糖活性的其他化合物。
