Department of Internal Medicine, Division of Nephrology, National Taiwan University Hospital Hsin-Chu Branch , 25, Ln. 442, Sec. 1, Jingguo Rd., 300 Hsinchu, Taiwan.
Department of Chemistry, National Taiwan Normal University , 162, Heping East Rd., Section 1, 106 Taipei, Taiwan.
ACS Sens. 2017 Dec 22;2(12):1788-1795. doi: 10.1021/acssensors.7b00564. Epub 2017 Nov 17.
In this work, a TFB (poly[(9,9-dioctylfluorenyl-2,7-diyl)-co-(4,4'-(N-(4-s-butylphenyl)diphenylamine)]) sensor with a cylindrical nanopore structure exhibits a high sensitivity to ammonia in ppb-regime. The lifetime and sensitivity of the TFB sensor were studied and compared to those of P3HT (poly(3-hexylthiophene)), NPB (N,N'-di(1-naphthyl)-N,N'-diphenyl-(1,1'-biphenyl)-4,4'-diamine), and TAPC (4,4'-cyclohexylidenebis[N,N-bis(4-methylphenyl) benzenamine]) sensors with the same cylindrical nanopore structures. The TFB sensor outstands the others in sensitivity and lifetime and it shows a sensing response (current variation ratio) of 13% to 100 ppb ammonia after 64 days of storage in air. A repeated sensing periods testing and a long-term measurement have also been demonstrated for the test of robustness. The performance of the TFB sensor is stable in both tests, which reveals that the TFB sensor can be utilized in our targeting clinical trials. In the last part of this work, we study the change of ammonia concentration in the breath of hemodialysis (HD) patients before and after dialysis. An obvious drop of breath ammonia concentration can be observed after dialysis. The reduction of breath ammonia is also correlated with the reduction of blood urea nitrogen (BUN). A correlation coefficient of 0.82 is achieved. The result implies that TFB sensor may be used as a real-time and low cost breath ammonia sensor for the daily tracking of hemodialysis patients.
在这项工作中，具有圆柱形纳米孔结构的 TFB（聚[（9,9-二辛基芴-2,7-二基）-共-（4,4'-(N-(4-仲丁基苯基)二苯基胺)）]）传感器对氨在 ppb 级表现出高灵敏度。研究了 TFB 传感器的寿命和灵敏度，并将其与 P3HT（聚（3-己基噻吩））、NPB（N,N'-二（1-萘基）-N,N'-二苯基-（1,1'-联苯）-4,4'-二胺）和 TAPC（4,4'-环己基二[N,N-双（4-甲基苯基）苯二胺））具有相同圆柱形纳米孔结构的传感器进行了比较。TFB 传感器在灵敏度和寿命方面优于其他传感器，在空气中储存 64 天后，对 100 ppb 氨的感应响应（电流变化比）为 13%。还进行了重复感应周期测试和长期测量，以测试其稳健性。在这两项测试中，TFB 传感器的性能都很稳定，这表明 TFB 传感器可用于我们的靶向临床试验。在这项工作的最后一部分，我们研究了血液透析（HD）患者透析前后呼吸中氨浓度的变化。透析后可以观察到呼吸中氨浓度明显下降。呼吸中氨的减少也与血液中尿素氮（BUN）的减少相关。达到了 0.82 的相关系数。结果表明，TFB 传感器可用作实时、低成本的呼吸氨传感器，用于日常跟踪血液透析患者。
