Marchis Daniela, Altomare Alessandra, Gili Marilena, Ostorero Federica, Khadjavi Amina, Corona Cristiano, Ru Giuseppe, Cappelletti Benedetta, Gianelli Silvia, Amadeo Francesca, Rumio Cristiano, Carini Marina, Aldini Giancarlo, Casalone Cristina
Istituto Zooprofilattico Sperimentale del Piemonte , Liguria e Valle D'Aosta, via Bologna 148, 10154 Torino, Italy.
Department of Pharmaceutical Sciences, Università degli Studi di Milano , Via Mangiagalli 25, 20133 Milano, Italy.
J Agric Food Chem. 2017 Dec 6;65(48):10638-10650. doi: 10.1021/acs.jafc.7b04639. Epub 2017 Nov 22.
An innovative analytical strategy has been applied to identify signature peptides able to distinguish among processed animal proteins (PAPs) derived from bovine, pig, fish, and milk products. Proteomics was first used to elucidate the proteome of each source. Starting from the identified proteins and using a funnel based approach, a set of abundant and well characterized peptides with suitable physical-chemical properties (signature peptides) and specific for each source was selected. An on-target LC-ESI-MS/MS method (MRM mode) was set up using standard peptides and was then applied to selectively identify the PAP source and also to distinguish proteins from bovine carcass and milk proteins. We believe that the method described meets the request of the European Commission which has developed a strategy for gradually lifting the "total ban" toward "species to species ban", therefore requiring official methods for species-specific discrimination in feed.
一种创新的分析策略已被应用于识别能够区分源自牛、猪、鱼和奶制品的加工动物蛋白（PAP）的特征肽。蛋白质组学首先用于阐明每种来源的蛋白质组。从已鉴定的蛋白质开始，并使用基于漏斗的方法，选择了一组具有合适物理化学性质的丰富且特征明确的肽（特征肽），且每种来源具有特异性。使用标准肽建立了一种靶向液相色谱 - 电喷雾串联质谱法（MRM模式），然后将其应用于选择性鉴定PAP来源，并区分牛胴体蛋白和乳蛋白。我们认为所描述的方法符合欧盟委员会的要求，该委员会已制定了一项战略，逐步将“全面禁令”转变为“按物种禁令”，因此需要用于饲料中物种特异性鉴别的官方方法。
