Lo S C, Liotta L A
Am J Pathol. 1985 Jan;118(1):7-13.
Patients with the acquired immunodeficiency syndrome (AIDS) have a high incidence of Kaposi's sarcoma. The etiology, histogenesis, and neoplastic nature of this neoplasm have been controversial. We have therefore searched for transforming gene(s) associated with AIDS Kaposi's sarcoma. DNA from an AIDS patient's Kaposi's sarcoma was transfected into NIH 3T3 cells. Control DNA was derived from human fibroblasts or salmon sperm. Kaposi's sarcoma DNA, but not the control DNA, transforms NIH/3T3 cells with a frequency of approximately 0.02 foci per 5 X 10(5) cells/micrograms DNA. The primary and secondary transfectants contain human repetitive DNA sequences. The transfected clones produced hemorrhagic angiosarcomatous neoplasms when implanted in nude mice. The histology of the nude mouse tumors is very similar to human Kaposi's sarcoma. The tumor produced by some transfectants is highly invasive and metastatic in nude mice. No significant homologues of rasN, rasH, rasK, v-sis, v-src and v-fes oncogenes (known to transform NIH/3T3 cells) were identified in the Kaposi's sarcoma DNA-transformed cells. Thus, AIDS Kaposi's sarcoma DNA may contain a distinct transforming gene(s).
获得性免疫缺陷综合征（艾滋病）患者卡波西肉瘤的发病率很高。这种肿瘤的病因、组织发生及肿瘤性质一直存在争议。因此，我们寻找了与艾滋病卡波西肉瘤相关的转化基因。将一名艾滋病患者卡波西肉瘤的DNA转染到NIH 3T3细胞中。对照DNA来源于人成纤维细胞或鲑鱼精子。卡波西肉瘤DNA而非对照DNA能以每5×10⁵个细胞/微克DNA约0.02个集落的频率转化NIH/3T3细胞。原代和二代转染细胞含有人类重复DNA序列。将转染克隆接种到裸鼠体内时会产生出血性血管肉瘤肿瘤。裸鼠肿瘤的组织学与人类卡波西肉瘤非常相似。一些转染细胞产生的肿瘤在裸鼠中具有高度侵袭性和转移性。在卡波西肉瘤DNA转化的细胞中未鉴定出rasN、rasH、rasK、v - sis、v - src和v - fes癌基因（已知可转化NIH/3T3细胞）的明显同源物。因此，艾滋病卡波西肉瘤DNA可能含有一种独特的转化基因。
