Parmaki Stella, Vyrides Ioannis, Vasquez Marlen I, Hartman Viki, Zacharia Irene, Hadjiadamou Ioanna, Barbeitos Catarina B M, Ferreira Frederico C, Afonso Carlos A M, Drouza Chrysoulla, Koutinas Michalis
Department of Environmental Science & Technology, Cyprus University of Technology, 30 Archbishop Kyprianou Str., 3036, Limassol, Cyprus.
Department of Chemistry, University of Cyprus, P.O. Box 20537, 1678, Nicosia, Cyprus.
Chemosphere. 2018 Feb;193:50-59. doi: 10.1016/j.chemosphere.2017.10.165. Epub 2017 Oct 30.
This work explores the potential for development of a lupanine valorization process evaluating different isolated microorganisms for their capacity to metabolize the alkaloid. Ecotoxicological assessment demonstrated that lupanine is toxic for Vibrio fischeri and Daphnia magna exhibiting EC values of 89 mg L and 47 mg L respectively, while acting both as growth inhibitor for a monocotyledonous and as promoter for a dicotyledonous plant. Among the eight aerobic and anaerobic strains isolated and identified Rhodococcus rhodochrous LPK211 achieved 81% removal for 1.5 g L lupanine, while no end-products were detected by NMR constituting a promising microorganism for lupanine biodegradation. Moreover, Rhodococcus ruber LPK111 and Rhodococcus sp. LPK311 exhibited 66% and 71% of removal respectively, including potential formation of lupanine N-oxide. Pseudomonas putida LPK411 reached 80% of lupanine removal and generated three fermentation products potentially comprising 17-oxolupanine and lupanine derivatives with open ring structures enabling the development of alkaloid valorization processes.
这项工作探索了开发一种羽扇豆碱增值工艺的潜力，评估了不同分离出的微生物代谢该生物碱的能力。生态毒理学评估表明，羽扇豆碱对费氏弧菌和大型溞有毒，其EC值分别为89毫克/升和47毫克/升，同时它既是单子叶植物的生长抑制剂，又是双子叶植物的生长促进剂。在分离和鉴定出的8株好氧和厌氧菌株中，红平红球菌LPK211对1.5克/升羽扇豆碱的去除率达到81%，而核磁共振未检测到终产物，这使其成为羽扇豆碱生物降解的一种有前景的微生物。此外，红球菌LPK111和红球菌属LPK311的去除率分别为66%和71%，包括可能形成羽扇豆碱N-氧化物。恶臭假单胞菌LPK411对羽扇豆碱的去除率达到80%，并产生了三种发酵产物，可能包括17-氧代羽扇豆碱和具有开环结构的羽扇豆碱衍生物，从而能够开发生物碱增值工艺。
