Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia; UMR-CNRS, Institute of Molecular Chemistry and Materials of Orsay, Bioorganic and Bioinorganic Chemistry Team, University Paris-Sud, Building 420, 91405 Orsay, France.
Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia.
Anal Chim Acta. 2017 Nov 22;994:10-18. doi: 10.1016/j.aca.2017.09.022. Epub 2017 Sep 22.
A new redox oligomer "oligo-methoxy-phenyl-acetonitrile" (Fc-acid-OMPA) modified with ferrocene groups and carboxylic acids as functional groups in side chains, has been synthesized. The redox indicator has been used for monitoring the electrochemical behavior. The functional groups give the flexibility for direct covalent attachment of biomolecules. The electrochemical properties of the redox oligomer film deposited on gold electrodes have been studied by cyclic voltammetry (CV), which showed a rate of electron transfer of 6.43 s. The oligomer has been studied as a transducer for electrochemical DNA sensing and for this purpose the acid functional group of Fc-acid-OMPA was attached with the DNA probe of hepatitis C bearing amino group in 5' position through amid link. The efficiency of DNA attachment on the oligomer film has been analyzed by X-Ray Photoelectron Spectrometry (XPS) and FT-IR spectroscopy. Cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS) have been used to analyze the biosensor construction and DNA detection. A wide linear range of detection from 1 fM to 100 pM have been demonstrated with a limit of detection (LOD) of 0.2 fM. The biosensor has showed an appreciated sensitivity to PCR samples of genomic DNA from Mycobacterium tuberculosis, and has been able to detect a single mutation which confers resistance of M. tuberculosis to rifampicin drug.
一种新型氧化还原低聚物“寡甲氧基苯乙腈”（Fc-酸-OMPA）已被合成。该低聚物带有侧链上的二茂铁基团和羧酸等功能基团。氧化还原指示剂已被用于监测电化学行为。功能基团赋予了直接共价连接生物分子的灵活性。通过循环伏安法（CV）研究了沉积在金电极上的氧化还原低聚物薄膜的电化学性质，结果表明电子转移速率为 6.43 s。该低聚物已被研究作为电化学 DNA 传感的传感器，为此，Fc-酸-OMPA 的酸性基团通过酰胺键与 5'位带有氨基的丙型肝炎 DNA 探针连接。X 射线光电子能谱（XPS）和傅里叶变换红外光谱（FT-IR）分析了低聚物薄膜上 DNA 附着的效率。循环伏安法（CV）和电化学阻抗谱（EIS）用于分析生物传感器的构建和 DNA 检测。该生物传感器具有从 1 fM 到 100 pM 的宽线性检测范围，检测限（LOD）为 0.2 fM。该生物传感器对结核分枝杆菌基因组 DNA 的 PCR 样本表现出较高的灵敏度，并且能够检测到导致结核分枝杆菌对利福平药物产生耐药性的单个突变。
