基于新型纳米复合换能器（Fc-ac-OMPA/MWCNTs）的结核分枝杆菌突变株的直接 E-DNA 传感器
Direct E-DNA sensor of Mycobacterium tuberculosis mutant strain based on new nanocomposite transducer (Fc-ac-OMPA/MWCNTs).
机构信息
Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia; UMR-CNRS, Institute of Molecular Chemistry and Materials of Orsay, Bioorganic and Bioinorganic Chemistry Team, University Paris-Sud, Building 420, 91405 Orsay, France.
Laboratory of Interfaces and Advanced Materials, Faculty of Science of Monastir, University of Monastir, 5019 Monastir, Tunisia.
出版信息
Talanta. 2018 Jul 1;184:475-483. doi: 10.1016/j.talanta.2018.03.025. Epub 2018 Mar 14.
Direct DNA sensor based on new nanocomposite materials (Fc-ac-OMPA/MWCNTs) has been investigated. This nanocomposite was formed by combining the redox oligomer "oligo-methoxy-phenyl-acetonitrile" (Fc-ac-OMPA) and the MWCNTs via a simple π-π stacking interaction in the aim to ameliorate the biosensor performance. The redox indicator and the functional groups of the redox oligomer have been used for monitoring the electrochemical behavior and the flexibility for direct covalent attachment of Hepatitis C DNA probe. This nanocomposite shows high performance of DNA hybridization with a detection limit of 0.08 fmol L. Moreover, the biosensor was applied for the detection of pathogenic bacterium such as DNA from Mycobacterium tuberculosis strand. Developed biosensor has been able to detect a single nucleotide polymorphism (SNP)T (TCG/ TTG) which confers resistance of M. tuberculosis to rifampicin drug.
基于新型纳米复合材料（Fc-ac-OMPA/MWCNTs）的直接 DNA 传感器已被研究。该纳米复合材料是通过在 MWCNTs 上结合氧化还原寡聚物“寡甲氧基苯乙腈”（Fc-ac-OMPA）形成的，通过简单的 π-π 堆积相互作用，旨在改善生物传感器的性能。氧化还原指示剂和氧化还原寡聚物的官能团已被用于监测电化学行为和直接共价附着丙型肝炎 DNA 探针的灵活性。这种纳米复合材料具有很高的 DNA 杂交性能，检测限为 0.08 fmol L。此外，该生物传感器还被用于检测分枝杆菌等病原体的 DNA 链。开发的生物传感器能够检测到单核苷酸多态性（SNP）T（TCG/TTG），该多态性赋予结核分枝杆菌对利福平药物的耐药性。
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