Le Tinh, Esteve-Turrillas Francesc A, Armenta Sergio, de la Guardia Miguel, Quiñones-Reyes Guillermo, Abad-Fuentes Antonio, Abad-Somovilla Antonio
Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain.
Department of Analytical Chemistry, University of Valencia, 50th Dr. Moliner St., 46100 Burjassot, Spain.
J Chromatogr A. 2017 Dec 22;1529:57-62. doi: 10.1016/j.chroma.2017.10.076. Epub 2017 Nov 1.
Specific monoclonal antibodies were coupled with magnetic Sepharose-based beads and used, for the first time. The methodology was applied to preconcentrate anatoxin-a from water and the later determination by ion mobility spectrometry (IMS). Dispersive magnetic immunoaffinity (d-MagIA) extraction methodology provided a limit of detection of 0.02μgL and a satisfactory precision with a relative standard deviation lower than 15%. Recoveries were evaluated at 0.5, 1.0 and 5.0μgL anatoxin-a with quantitative values from 91 to 115%. Additionally, isobaric interferences with phenylalanine were completely avoided by the use of the developed d-MagIA extraction coupled to IMS determinations.
特异性单克隆抗体首次与基于磁珠的琼脂糖结合并使用。该方法用于从水中预浓缩anatoxin-a，随后通过离子迁移谱（IMS）进行测定。分散磁免疫亲和（d-MagIA）萃取方法的检测限为0.02μg/L，相对标准偏差低于15%，具有令人满意的精密度。在anatoxin-a浓度为0.5、1.0和5.0μg/L时评估回收率，定量值为91%至115%。此外，通过使用开发的d-MagIA萃取与IMS测定相结合，完全避免了与苯丙氨酸的等压干扰。
