Price Institute of Surgical Research, University of Louisville, Louisville, KY, USA; Cellular and Molecular Biology Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Department of Cell & Molecular Medicine, (Formerly, Anatomy and Cell Biology), Rush University Medical Center, 1750 W. Harrison St., Suite 1413A, Chicago Il 60612, USA.
Prog Biophys Mol Biol. 2018 Mar;133:36-48. doi: 10.1016/j.pbiomolbio.2017.11.001. Epub 2017 Nov 8.
To compare the in vitro effectiveness of Low-Level Laser Therapy (LLLT) and Low Intensity Pulsed Ultrasound (LIPUS) on bony cells and related stem cells. In this study, we aim to systematically review the published scientific literature which explores the use of LLLT and LIPUS to biostimulate the activity or the proliferation of bony cells or stem cells in vitro. We searched the database PubMed for LLLT or LIPUS, with/without bone, osteoblast, osteocyte, stem cells, the human osteosarcoma cell line (MG63), bone-forming cells, and cell culture (or in vitro). These studies were subdivided into categories exploring the effect of LLLT or LIPUS on bony cells, stem cells, and other related cells. 75 articles were found between 1987 and 2016; these included: 50 full paper articles on LLLT and 25 full papers on LIPUS. These articles met the eligibility criteria and were included in our review. A detailed and concise description of the LLLT and the LIPUS protocols and their individual effects on bony cells or stem cells and their results are presented in five tables. Based on the main results and the conclusions of the reviewed articles in the current work, both, LLLT and LIPUS, apply a biostimulatory effect on osteoblasts, osteocytes, and enhance osteoblast proliferation and differentiation on different bony cell lines used in in vitro studies, and therefore, these may be useful tools for bone regeneration therapy. Moreover, in consideration of future cell therapy protocols, both, LLLT and LIPUS (especially LLLT), enhnce a significant increase in the initial number of SCs before differentiation, thus increasing the number of differentiated cells for tissue engineering, regenerative medicine, and healing. Further studies are necessary to determine the LLLT or the LIPUS parameters, which are optimal for biostimsulating bony cells and SCs for bone healing and regenerative medicine.
比较低水平激光疗法（LLLT）和低强度脉冲超声（LIPUS）对骨细胞和相关干细胞的体外有效性。在这项研究中，我们旨在系统地回顾已发表的科学文献，这些文献探讨了 LLLT 和 LIPUS 用于体外刺激骨细胞或干细胞活性或增殖的用途。我们在 PubMed 数据库中搜索了 LLLT 或 LIPUS 与/或骨、成骨细胞、骨细胞、干细胞、人骨肉瘤细胞系（MG63）、成骨细胞和细胞培养（或体外）的相关文献。这些研究分为探讨 LLLT 或 LIPUS 对骨细胞、干细胞和其他相关细胞影响的类别。1987 年至 2016 年共发现 75 篇文章；其中包括：50 篇关于 LLLT 的全文文章和 25 篇关于 LIPUS 的全文文章。这些文章符合入选标准并被纳入我们的综述。在五张表中详细而简洁地描述了 LLLT 和 LIPUS 的方案及其对骨细胞或干细胞的单独作用及其结果。基于当前工作中综述文章的主要结果和结论，LLLT 和 LIPUS 都对成骨细胞、骨细胞产生生物刺激作用，并增强不同体外研究中使用的骨细胞系中成骨细胞的增殖和分化，因此，这些可能是骨再生治疗的有用工具。此外，考虑到未来的细胞治疗方案，LLLT 和 LIPUS（尤其是 LLLT）都会在分化前显著增加 SCs 的初始数量，从而增加组织工程、再生医学和愈合的分化细胞数量。需要进一步的研究来确定 LLLT 或 LIPUS 参数，这些参数对于生物刺激骨细胞和 SCs 以促进骨愈合和再生医学是最优的。
