Department of Medical Biochemistry, Faculty of Medicine, Marmara University, Istanbul 34854, Turkey; Genetic and Metabolic Diseases Research Center (GEMHAM), Marmara University, Istanbul 34854, Turkey.
Department of Medical Biochemistry, Faculty of Medicine, Marmara University, Istanbul 34854, Turkey; Genetic and Metabolic Diseases Research Center (GEMHAM), Marmara University, Istanbul 34854, Turkey.
Clin Nutr. 2018 Dec;37(6 Pt A):2166-2171. doi: 10.1016/j.clnu.2017.10.016. Epub 2017 Nov 1.
Walnuts contain many components including specific fatty acids, which could be active against cancer. Even though the anticarcinogenic effect of some of the individual fatty acids in walnut oil has been described, the effect of walnut oil itself on esophageal cancer cells hasn't yet been investigated.
We aimed to investigate whether walnut oil affects tumor growth and metastatic potential in esophageal cancer cells.
The human esophageal adenocarcinoma cell line, OE19, was treated with different doses of walnut oil and cell viability, apoptosis/necrosis and cell cycle analyses were performed using WST-1 assay and flow cytometry respectively. Adhesion, colony formation and wound healing assays were performed to assess the antimetastatic effects of walnut oil. NFkB expression was evaluated with western blot analysis.
Walnut oil decreased the cell viability of esophageal cancer cells in a dose-dependent manner. 20 mg/mL walnut oil reduced cell viability by ∼50% when compared with control. The analysis revealed that necrosis and accumulation of cells in G0/G1 phase was induced in the cells treated with high doses of walnut oil. It also down-regulated the protein levels of NFkB. Walnut oil suppressed the adhesion, migration and colony formation of the cells.
High-dose short-term administration of walnut oil reduces the cell viability and metastatic ability of esophageal cancer cells, while exhibiting anticarcinogenic effect by inducing necrosis and cell cycle arrest at the G0/G1 phase, probably through suppression of the NFkB pathway. These data indicate that walnut oil, and by extension walnut consumption, may have beneficial effects in esophageal cancer in humans. This should be tested by clinical trials in the future.
核桃含有多种成分，包括特定的脂肪酸，这些成分可能具有抗癌作用。尽管已经描述了核桃油中某些脂肪酸的抗癌作用，但核桃油本身对食管癌细胞的影响尚未得到研究。
我们旨在研究核桃油是否会影响食管癌细胞的肿瘤生长和转移潜能。
用不同剂量的核桃油处理人食管腺癌细胞系 OE19，分别用 WST-1 检测法和流式细胞术检测细胞活力、细胞凋亡/坏死和细胞周期分析。通过黏附、集落形成和划痕愈合实验来评估核桃油的抗转移作用。用 Western blot 分析评估 NFkB 表达。
核桃油呈剂量依赖性降低食管癌细胞的细胞活力。与对照组相比，20mg/mL 的核桃油使细胞活力降低了约 50%。分析表明，高剂量的核桃油处理会诱导细胞坏死和积累在 G0/G1 期。它还下调了 NFkB 蛋白的水平。核桃油抑制了细胞的黏附、迁移和集落形成。
高剂量短期给予核桃油可降低食管癌细胞的细胞活力和转移能力，同时通过诱导细胞坏死和细胞周期停滞在 G0/G1 期发挥抗癌作用，可能通过抑制 NFkB 通路。这些数据表明，核桃油，以及更广泛地说，核桃的摄入，可能对人类食管癌有有益的影响。这应该在未来的临床试验中进行测试。
