D'Angeli Floriana, Malfa Giuseppe Antonio, Garozzo Adriana, Li Volti Giovanni, Genovese Carlo, Stivala Aldo, Nicolosi Daria, Attanasio Francesco, Bellia Francesco, Ronsisvalle Simone, Acquaviva Rosaria
Department of Biomedical and Biotechnological Sciences, Section of Biochemistry, University of Catania, 95123 Catania, Italy.
Department of Human Sciences and Quality of Life Promotion, San Raffaele Roma Open University, 00166 Rome, Italy.
Antibiotics (Basel). 2021 Feb 4;10(2):159. doi: 10.3390/antibiotics10020159.
The difficulty to treat resistant strains-related hospital-acquired infections (HAIs) promoted the study of phytoextracts, known sources of bioactive molecules. Accordingly, in the present study, the pharmacological activities of (L.) pellicle extract (WPE) were investigated. The antiviral effect was tested against virus type 1 and 2, 1, 2, 9, B1 through the plaque reduction assay. The antibacterial and antifungal activities were evaluated against medically important strains, by the microdilution method. DPPH and superoxide dismutase (SOD)s-like activity assays were used to determine the antioxidant effect. Besides, the extract was screened for cytotoxicity on Caco-2, MCF-7, and HFF1 cell lines by the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. The total phenolic and flavonoid contents were also evaluated. Interestingly, WPE inhibited viruses (HSVs) replication, bacterial and fungal growth. WPE showed free radical scavenging capacity and inhibited superoxide anion formation in a dose-dependent manner. These effects could be attributed to the high content of phenols and flavonoids, which were 0.377 ± 0.01 mg GE/g and 0.292 ± 0.08 mg CE/g, respectively. Moreover, WPE was able to reduce Caco-2 cell viability, at both 48 h and 72 h. The promising results encourage further studies aimed to better elucidate the role of WPE in the prevention of human infectious diseases.
治疗与耐药菌株相关的医院获得性感染（HAIs）的困难推动了对植物提取物的研究，植物提取物是已知的生物活性分子来源。因此，在本研究中，对（L.）菌膜提取物（WPE）的药理活性进行了研究。通过蚀斑减少试验测试了其对1型和2型单纯疱疹病毒、1型、2型、9型、B1型病毒的抗病毒效果。通过微量稀释法评估了其对医学上重要菌株的抗菌和抗真菌活性。采用二苯基苦味酰基自由基（DPPH）和超氧化物歧化酶（SOD）样活性测定法来确定抗氧化效果。此外，通过3-[4,5-二甲基噻唑-2-基]-2,5-二苯基四氮唑溴盐（MTT）试验筛选了该提取物对Caco-2、MCF-7和HFF1细胞系的细胞毒性。还评估了总酚和黄酮含量。有趣的是，WPE抑制了单纯疱疹病毒（HSVs）复制、细菌和真菌生长。WPE表现出自由基清除能力，并以剂量依赖方式抑制超氧阴离子形成。这些作用可能归因于酚类和黄酮类的高含量，其含量分别为0.377±0.01毫克没食子酸当量/克和0.292±0.08毫克儿茶素当量/克。此外，WPE在48小时和72小时时均能够降低Caco-2细胞活力。这些有前景的结果鼓励开展进一步研究，以更好地阐明WPE在预防人类传染病中的作用。
