体外精子穿透激素标准化的人宫颈黏液的预后价值。

Prognostic value of in vitro sperm penetration into hormonally standardized human cervical mucus.

作者信息

Eggert-Kruse W, Leinhos G, Gerhard I, Tilgen W, Runnebaum B

机构信息

Division of Gynecological Endocrinology, Women's Hospital, University of Heidelberg, West Germany.

出版信息

Fertil Steril. 1989 Feb;51(2):317-23. doi: 10.1016/s0015-0282(16)60497-1.

DOI:10.1016/s0015-0282(16)60497-1

PMID:2912776

Abstract

To analyze the prognostic value of the sperm cervical mucus penetration test (SCMPT), fresh semen samples of 99 male patients under infertility investigation were exposed to capillary tubes filled with freshly obtained cervical mucus (CM) of the patients' wives (WCM), fertile donors (DCM), and bovine CM (BCM). The quality of the human CM was standardized by oral administration of estrogens. The overall pregnancy rate after 6 months was 17.2% (17/99), and was significantly different in couples with poor and good SCMPT with WCM (1/44, 2.3% versus 16/55, 29%; P less than 0.001) in a prospective study. Human CM was superior to BCM as a penetration medium in providing more information about sperm function. The results suggest that in vitro sperm penetration testing with hormonally standardized CM of female partners adds an important dimension to sperm analysis with regard to fertility prognosis.

摘要

为分析精子-宫颈黏液穿透试验（SCMPT）的预后价值，对99名接受不育症检查的男性患者的新鲜精液样本进行检测，将其暴露于装有患者妻子新鲜宫颈黏液（WCM）、可育供者宫颈黏液（DCM）和牛宫颈黏液（BCM）的毛细管中。通过口服雌激素使人类宫颈黏液质量标准化。在一项前瞻性研究中，6个月后的总体妊娠率为17.2%（17/99），使用WCM时，SCMPT结果差和结果好的夫妇的妊娠率有显著差异（1/44，2.3%对16/55，29%；P<0.001）。作为穿透介质，人类宫颈黏液在提供更多精子功能信息方面优于牛宫颈黏液。结果表明，使用经激素标准化的女性伴侣宫颈黏液进行体外精子穿透试验，在生育预后方面为精子分析增添了重要内容。