流式细胞术分析急性髓系白血病免疫表型
Acute Myeloid Leukemia Immunophenotyping by Flow Cytometric Analysis.
作者信息
Chen Xueyan, Cherian Sindhu
机构信息
Department of Laboratory Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA.
Department of Laboratory Medicine, University of Washington, 1959 NE Pacific Street, Seattle, WA 98195, USA.
出版信息
Clin Lab Med. 2017 Dec;37(4):753-769. doi: 10.1016/j.cll.2017.07.003. Epub 2017 Aug 31.
Flow cytometry plays an indispensible role in the diagnosis and subclassification of acute myeloid leukemia (AML). Using a multiparametric approach, flow cytometry immunophenotyping has the advantage of efficiency with high sensitivity. This article reviews the general gating strategy, antibody panels for routine analysis, and additional markers for lineage assignment in the subclassification of AML. Also discussed are diagnostic immunophenotypic features of hard-to-classify entities considered within the differential diagnosis of AML. Finally, briefly presented are the principles underlying the use of flow cytometry for minimal residual disease detection.
流式细胞术在急性髓系白血病（AML）的诊断和亚分类中发挥着不可或缺的作用。采用多参数方法，流式细胞术免疫表型分析具有高效且灵敏度高的优势。本文综述了AML亚分类中的一般设门策略、常规分析的抗体组合以及用于谱系确定的其他标志物。还讨论了AML鉴别诊断中难以分类实体的诊断性免疫表型特征。最后，简要介绍了流式细胞术用于微小残留病检测的基本原理。
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