Rana Kavita, Verma Yeshvandra, Rani Varsha, Rana Suresh Vir Singh
Toxicology Laboratory, Department of Zoology/Toxicology, Chaudhary Charan Singh University, Meerut, 250004, India.
Toxicology Laboratory, Department of Zoology/Toxicology, Chaudhary Charan Singh University, Meerut, 250004, India.
Chemosphere. 2018 Feb;193:142-150. doi: 10.1016/j.chemosphere.2017.11.011. Epub 2017 Nov 4.
During present investigations, renal toxicity of CdSNPs (cadmium sulphide nanoparticles) (ranged 5-9 nm) was estimated in rat employing specific parameters. Treatment on each alternate day for 45 days with CdSNPs (10 mg/kg b.w.) yielded significant increase in Cd-MT (cadmium metallothionein), lipid peroxidation and HO generation in the kidney of rat in comparison to bulk cadmium. Concentration of creatinine also increased in urine of CdSNPs treated rats. Histopathological observations revealed extensive damage in proximal tubules. Ultrastructural studies showed mitochondrial, nuclear and ER (endoplasmic reticulum) changes. Finally, renal toxicity of CdSNPs was confirmed by loss of alkaline phosphatase from the brush border of proximal convoluted tubules. These observations conclude that CdSNPs manifest greater toxicity in kidney than cadmium sulphide bulk particles. These effects have been attributed to their specific physicochemical properties, greater potential to generate ROS and induction of oxidative stress and impairment of renal structure and function. Present observations suggest that commercial/industrial use of CdSNPs may pose serious renal health problems in man.
在目前的研究中，使用特定参数在大鼠中评估了硫化镉纳米颗粒（CdSNPs）（粒径范围为5 - 9纳米）的肾毒性。与块状镉相比，用CdSNPs（10毫克/千克体重）每隔一天处理大鼠45天，大鼠肾脏中的镉 - 金属硫蛋白（Cd - MT）、脂质过氧化和羟基（HO）生成显著增加。CdSNPs处理的大鼠尿液中的肌酐浓度也有所增加。组织病理学观察显示近端小管有广泛损伤。超微结构研究显示线粒体、细胞核和内质网（ER）发生变化。最后，通过近端曲管刷状缘碱性磷酸酶的丧失证实了CdSNPs的肾毒性。这些观察结果得出结论，CdSNPs在肾脏中表现出比硫化镉块状颗粒更大的毒性。这些影响归因于它们特定的物理化学性质、产生活性氧的更大潜力以及氧化应激的诱导以及肾脏结构和功能的损害。目前的观察结果表明，CdSNPs的商业/工业用途可能会给人类带来严重的肾脏健康问题。
