El-Nahass Eman E, Salem B I, El-Naggar Sabry A, Elwan Mona M
Zoology Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Physics Department, Faculty of Science, Tanta University, Tanta, 31527, Egypt.
Sci Rep. 2025 Feb 26;15(1):6903. doi: 10.1038/s41598-025-90043-x.
Cobalt Zinc ferrite nanoparticles (NPs) were synthesized utilizing the auto-combustion flash method, with the general formula CoZnFeO (x = 0,0.35). This study aimed to evaluate the hepato-renal and systemic toxicity of Cobalt Zinc Ferrite nanoparticles (CZF NPs). A total of eighty female mice were utilized to ascertain the median lethal dose (LD50) of CF NPs (100 mg/kg) and CZF NPs (100 mg/kg). Thirty female CD1 mice were placed into three groups, each containing ten animals. In Group 1 (Gp1), mice were administered a 200 µl injection of sterile saline intraperitoneally (i.p.). During a 6-day period, Gp2 and Gp3 received injections of CF NPs and CFZ NPs. On day 14 after injection, hematological, biochemical, and histopathological data were measured. CZF NPs were characterized using X-ray Diffraction Analysis (XRD), Transmission Electron Microscope (TEM) and Vibrating Sample Magnetometer (VSM). There was a significant alteration in the overall body weight of mice injected with CZF NPs. Injections of CF NPs did not significantly alter red blood cells (RBC) counts, hemoglobin concentration (Hb), hematocrit percentage (Hct%), total white blood cells (WBCs), and platelets. However, injections of CZF NPs resulted in an increase in WBC count and a decrease in platelet count. Furthermore, injection of CZF NPs altered the differential leukocyte percentages. The liver and kidney functions in mice injected with CF NPs did not show any notable changes. However, mice treated with CZF NPs had considerable increases in liver and kidney bio-markers. The administration of CF NPS did not modify the histological structure of hepatic and renal tissues; however, the hepatic and renal structures were disrupted in animals injected with CZF NPs. Overall, the findings indicated high toxicity of CZF NPs in the mice used for the experiment.
采用自动燃烧闪蒸法合成了通式为CoZnFeO（x = 0,0.35）的钴锌铁氧体纳米颗粒（NPs）。本研究旨在评估钴锌铁氧体纳米颗粒（CZF NPs）的肝肾毒性和全身毒性。总共使用了80只雌性小鼠来确定CF NPs（100 mg/kg）和CZF NPs（100 mg/kg）的半数致死剂量（LD50）。将30只雌性CD1小鼠分为三组，每组10只。在第1组（Gp1）中，小鼠腹腔内（i.p.）注射200 μl无菌盐水。在6天期间，Gp2和Gp3分别接受CF NPs和CFZ NPs注射。注射后第14天，测量血液学、生化和组织病理学数据。使用X射线衍射分析（XRD）、透射电子显微镜（TEM）和振动样品磁强计（VSM）对CZF NPs进行了表征。注射CZF NPs的小鼠总体体重有显著变化。注射CF NPs对红细胞（RBC）计数、血红蛋白浓度（Hb）、血细胞比容百分比（Hct%）、总白细胞（WBCs）和血小板没有显著影响。然而，注射CZF NPs导致白细胞计数增加和血小板计数减少。此外，注射CZF NPs改变了白细胞分类百分比。注射CF NPs的小鼠肝脏和肾脏功能没有显示出任何明显变化。然而，用CZF NPs处理的小鼠肝脏和肾脏生物标志物有相当大的增加。给予CF NPS没有改变肝脏和肾脏组织的组织结构；然而，注射CZF NPs的动物肝脏和肾脏结构受到破坏。总体而言，研究结果表明CZF NPs对用于实验的小鼠具有高毒性。
