Huidobro Nayeli, De la Torre-Valdovinos Braniff, Mendez Abraham, Treviño Mario, Arias-Carrion Oscar, Chavez Fermin, Gutierrez Ranier, Manjarrez Elias
Instituto de Fisiología, Benemérita Universidad Autónoma de Puebla, 14 Sur 6301, Col. San Manuel, Apartado Postal 406, Puebla, Pue. CP 72570, México.
Departamento de Ciencias Computacionales, CUCEI, Universidad de Guadalajara, México.
Neurosci Lett. 2018 Jan 18;664:51-57. doi: 10.1016/j.neulet.2017.11.004. Epub 2017 Nov 9.
We examined whether the optogenetic noise-photostimulation (ONP) of the barrel cortex (BC) of anesthetized Thy1-ChR2-YFP transgenic mice increases the neuronal multiunit-activity response evoked by whisker mechanical stimulation (whisker-evoked MUA). In all transgenic mice, we found that the signal-to-noise ratio (SNR) of such whisker-evoked MUA signals exhibited an inverted U-like shape as a function of the ONP level. Numerical simulations of a ChR2-expressing neuron model qualitatively support our experimental data. These results show that the application of an intermediate intensity of ONP in the brain can increase cortical somatosensory spike responses to whisker protraction. These findings suggest that ONP of the mice-BC could produce improvements in somatosensory perception to whisker stimulation.
我们研究了对麻醉的Thy1-ChR2-YFP转基因小鼠的桶状皮层（BC）进行光遗传学噪声光刺激（ONP）是否会增加触须机械刺激诱发的神经元多单位活动反应（触须诱发的MUA）。在所有转基因小鼠中，我们发现这种触须诱发的MUA信号的信噪比（SNR）作为ONP水平的函数呈倒U形。表达ChR2的神经元模型的数值模拟定性地支持了我们的实验数据。这些结果表明，在大脑中施加中等强度的ONP可以增加皮层体感尖峰对触须前伸的反应。这些发现表明，小鼠BC的ONP可以改善对触须刺激的体感感知。
