College of Veterinary Medicine, Chungnam National University, Yuseong-gu, Daejeon 34134, Republic of Korea.
Department of Mechanical Engineering, Korea Advanced Institute of Science and Technology, 291 Daehak-ro, Yuseong-gu, Daejeon 34141, Republic of Korea.
Int J Biol Macromol. 2018 Mar;108:1281-1288. doi: 10.1016/j.ijbiomac.2017.11.046. Epub 2017 Nov 10.
In this study, we investigated the antifungal activity and cytotoxicity of ZnO-chitosan nanocomposites (ZnO-C NCs) against Candida albicans and human epithelial type 2 (HEp2) cells, respectively. The crystalline phase, morphology, composition, particle size and optical absorption properties of the synthesized ZnO-C NCs were systematically investigated by various contemporary methods. The X-ray diffraction analysis results showed characteristic diffraction peaks corresponding to both ZnO and chitosan, while field-emission scanning electron microscopy (FESEM) displayed clusters of spherical shaped particulate morphology. UV-vis absorption spectra showed a shift in the optical absorption towards lower wavelength for ZnO-C NCs when compared to ZnO nanoparticles (NPs). The antifungal activity results (against C. albicans) showed that the minimum inhibitory concentration of ZnO NPs and ZnO-C NCs were 200μg/mL and 75μg/mL, respectively, suggesting the greater therapeutic potential of ZnO-C NCs. FESEM analysis results showed the substantial change in the external morphology of C. albicans after treatment with both ZnO NPs and ZnO-C NCs due to the fungal cell membrane damage. ZnO-C NCs displayed lower cytotoxicity with HEp2 cells indicating the good cytocompatibility of the synthesized ZnO-C NCs. It is expected that ZnO and chitosan complement each other and exhibit synergistic effects potential for antimicrobial and biomedical applications.
在这项研究中，我们分别研究了氧化锌-壳聚糖纳米复合材料（ZnO-C NCs）对白色念珠菌和人上皮细胞 2 型（HEp2）的抗真菌活性和细胞毒性。通过各种现代方法系统地研究了合成的 ZnO-C NCs 的晶体相、形态、组成、粒径和光吸收特性。X 射线衍射分析结果表明，产物既具有 ZnO 的特征衍射峰，又具有壳聚糖的特征衍射峰，而场发射扫描电子显微镜（FESEM）则显示出球形颗粒形态的团聚体。紫外可见吸收光谱显示，与 ZnO 纳米颗粒（NPs）相比，ZnO-C NCs 的光吸收向低波长方向移动。抗真菌活性结果（针对白色念珠菌）表明，ZnO NPs 和 ZnO-C NCs 的最小抑菌浓度分别为 200μg/mL 和 75μg/mL，这表明 ZnO-C NCs 具有更大的治疗潜力。FESEM 分析结果表明，由于真菌细胞膜的损伤，白色念珠菌的外部形态在 ZnO NPs 和 ZnO-C NCs 处理后发生了显著变化。ZnO-C NCs 对 HEp2 细胞的细胞毒性较低，表明合成的 ZnO-C NCs 具有良好的细胞相容性。预计 ZnO 和壳聚糖可以相互补充，并表现出协同作用，具有抗菌和生物医学应用的潜力。
