Youssef Ahmed M, El-Nahrawy Amany M, Abou Hammad Ali B
Packing and Packaging Materials Department, National Research Centre, 33 El Bohouth St.(former El Tahrir st.), Dokki, Giza, P.O. 12622, Egypt.
Solid State Physics Department, National Research Centre, 33 El Bohouth St.(former El Tahrir st.), Dokki, Giza, P.O. 12622, Egypt.
Int J Biol Macromol. 2017 Apr;97:561-567. doi: 10.1016/j.ijbiomac.2017.01.059. Epub 2017 Jan 17.
Hybrid Chitosan/Poly ethylene glycol/calcium silicate (CS/PEG/calcium silicate) nanocomposite modified with different two types, zinc oxide nanoparticles (ZnO-NPs) and tartrazine dye (E102) were prepared by sol gel method and the characterization of their structure and biological properties were carried out in order to evaluate the possible use in optical and biomedical fields. The hybrid CS/PEG/calcium silicate complex formations have been established by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopic analysis. The spheres-like chitosan-PEG/calcium silicate and modified with both ZnO-NPs and (E102) were obtained with optimum concentration of 11% ZnO-NPs and 0.3gm (E102) dyes. Spheres-like particle shape of these nanocomposites from SEM images, higher UV absorption in the region of 200-300nm by UV-vis absorption spectrophotometer are recorded. The fabricate CS/PEG/calcium silicate nanocomposites and doped with ZnO-NPs and tetrazine were studied contrary to gram positive (Staphylococcus aureus), gram negative (Pseudomonas aeruginosa) bacteria, fungi (Candidia albicans) and Aspargillus niger via the agar plate method. The obtained results indicated that the prepared CS-PEG/calcium silicate nanocomposites have good antibacterial properties agnist G, G bacteria and fungi, so that it could be a promised candidate in various optical and in biological applications as well as packaging application.
采用溶胶 - 凝胶法制备了用两种不同类型的氧化锌纳米颗粒（ZnO-NPs）和酒石黄染料（E102）改性的壳聚糖/聚乙二醇/硅酸钙（CS/PEG/硅酸钙）杂化纳米复合材料，并对其结构和生物学特性进行了表征，以评估其在光学和生物医学领域的潜在应用。通过X射线衍射（XRD）和傅里叶变换红外（FTIR）光谱分析确定了杂化CS/PEG/硅酸钙复合物的形成。以11%的ZnO-NPs和0.3克（E102）染料的最佳浓度获得了同时用ZnO-NPs和（E102）改性的球状壳聚糖 - PEG/硅酸钙。从扫描电子显微镜（SEM）图像可以看出这些纳米复合材料呈球状颗粒形状，用紫外可见吸收分光光度计记录到在200 - 300nm区域有较高的紫外线吸收。通过琼脂平板法研究了制备的CS/PEG/硅酸钙纳米复合材料以及掺杂了ZnO-NPs和酒石黄的纳米复合材料对革兰氏阳性菌（金黄色葡萄球菌）、革兰氏阴性菌（铜绿假单胞菌）、真菌（白色念珠菌）和黑曲霉的抗菌性能。所得结果表明，制备的CS-PEG/硅酸钙纳米复合材料对革兰氏阳性菌、革兰氏阴性菌和真菌具有良好的抗菌性能，因此它有望成为各种光学、生物应用以及包装应用中的候选材料。
