波兰体外克林霉素对拟杆菌属和副拟杆菌属分离株的作用。
In vitro effect of clindamycin against Bacteroides and Parabacteroides isolates in Poland.
机构信息
Department of Medical Microbiology, Medical University of Warsaw, Chalubinskiego 5 Str., 02-004 Warsaw, Poland; Department of Medical Microbiology, The Infant Jesus Teaching Hospital, Lindleya 4 Str., 02-005 Warsaw, Poland.
Department of Medical Microbiology, Medical University of Warsaw, Chalubinskiego 5 Str., 02-004 Warsaw, Poland; Department of Medical Microbiology, The Infant Jesus Teaching Hospital, Lindleya 4 Str., 02-005 Warsaw, Poland.
出版信息
J Glob Antimicrob Resist. 2018 Jun;13:49-52. doi: 10.1016/j.jgar.2017.11.001. Epub 2017 Nov 10.
OBJECTIVES
The aims of this study were (i) to analyse strains of the genera Bacteroides and Parabacteroides isolated from clinical specimens for phenotypic resistance to clindamycin, (ii) to detect erm genes in the isolates and (iii) to determine any correlation between in vitro resistance and the presence of erm genes.
METHODS
The Bacteroides and Parabacteroides isolates analysed were obtained from patients hospitalised at teaching hospitals in Poland. Antimicrobial susceptibility testing was performed by Etest and the results were interpreted according to European Committee on Antimicrobial Susceptibility Testing (EUCAST) guidelines. All isolates were analysed by PCR for the presence of the resistance genes ermF, ermB and ermG.
RESULTS
Resistance to clindamycin was detected in 31.0% (62/200) of all evaluated isolates, with the ermF and ermB genes detected in 31.0% (62/200) and 0.5% (1/200) of isolates, respectively. No isolates with ermG were detected among the evaluated strains. Pearson's test showed an almost perfect correlation between clindamycin minimum inhibitory concentrations (MICs) and the presence of ermF in Bacteroides spp. and Parabacteroides distasonis isolates, although the ermF gene was also present in 10 clindamycin-susceptible isolates of Bacteroides spp.
CONCLUSIONS
This study demonstrated a substantial proportion of Bacteroides (22.5-100% depending on the species) and 50.0% of Parabacteroides strains exhibiting resistance to clindamycin. The clindamycin MIC for resistant strains in each case was ≥256mg/L. Resistance to clindamycin in Bacteroides and Parabacteroides species is correlated mainly with the presence of the ermF gene.
目的
本研究的目的是(i) 分析从临床标本中分离的拟杆菌属和副拟杆菌属菌株对克林霉素的表型耐药性，(ii) 检测分离株中的 erm 基因，以及(iii) 确定体外耐药性与 erm 基因存在之间的任何相关性。
方法
分析的拟杆菌属和副拟杆菌属分离株来自波兰教学医院住院患者。通过 Etest 进行抗菌药物敏感性试验，结果根据欧洲抗菌药物敏感性试验委员会 (EUCAST) 指南进行解释。所有分离株均通过 PCR 分析 ermF、ermB 和 ermG 耐药基因的存在情况。
结果
在所评估的所有 200 株分离株中，有 31.0%（62/200）对克林霉素耐药，其中 ermF 和 ermB 基因分别在 31.0%（62/200）和 0.5%（1/200）的分离株中检出。在所评估的菌株中未检出 ermG 基因。Pearson 检验显示，在拟杆菌属和副拟杆菌属分离株中，克林霉素最低抑菌浓度 (MIC) 与 ermF 的存在之间存在几乎完美的相关性，尽管 ermF 基因也存在于 10 株克林霉素敏感的拟杆菌属分离株中。
结论
本研究表明，相当一部分拟杆菌属（22.5-100%，具体取决于物种）和 50.0%的副拟杆菌属菌株对克林霉素耐药。每种情况下耐药菌株的克林霉素 MIC 均≥256mg/L。拟杆菌属和副拟杆菌属对克林霉素的耐药性主要与 ermF 基因的存在相关。
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