检测拟杆菌属和副拟杆菌属菌株中的耐药基因和药敏模式。
Detection of resistance genes and susceptibility patterns in Bacteroides and Parabacteroides strains.
机构信息
Departamento de Microbiologia Médica, Instituto de Microbiologia Prof. Paulo de Góes/UFRJ, Laboratório de Biologia de Anaeróbios, Centro de Ciências da Saúde, Ilha do Fundão, Cidade Universitária, Rio de Janeiro/RJ 21941-902, Brasil.
出版信息
Anaerobe. 2010 Jun;16(3):190-4. doi: 10.1016/j.anaerobe.2010.02.003. Epub 2010 Feb 14.
Susceptibility to five antimicrobials was determined for Bacteroides spp. (n = 52) and Parabacteroides distasonis (n = 8). All isolates were susceptible to metronidazole. The resistance rates to ampicillin, cefoxitin, tetracycline and clindamycin were 98%, 9.6%, 65.3% and 19.2% of the Bacteroides strains, respectively. The genes cepA, cfiA, cfxA, tetQ, ermF and nim were found in 69.2%, 17.3% 9.6%, 50%, 7.7% and 3.8% for these strains respectively. All P. distasonis strains were resistant to ampicilin. Cefoxitin, tetracycline and clindamycin resistance rates were 75%, 87.5% and 50%, respectively. The ermF and nim genes were absent and 37.5%, 12.5%, 12.5% and 87.5% of this strains possessed cepA, cfiA, cfxA and tetQ genes, respectively. Ten cfiA gene positive strains of Bacteroides and Parabacteroides were submitted to E-test with imipenem and amoxicillin-clavulanate. The resistance rate to imipenem was 4.1% and 8.3% to amoxicillin-clavulanate. This feature is for the first time described in Brazil.
测定了 52 株拟杆菌属（Bacteroides spp.）和 8 株副拟杆菌（Parabacteroides distasonis）对 5 种抗菌药物的敏感性。所有分离株均对甲硝唑敏感。氨苄西林、头孢西丁、四环素和克林霉素的耐药率分别为 98%、9.6%、65.3%和 19.2%。在这些菌株中，分别发现了 cepA、cfiA、cfxA、tetQ、ermF 和 nim 基因，占 69.2%、17.3%、9.6%、50%、7.7%和 3.8%。所有副拟杆菌属菌株均对氨苄西林耐药。头孢西丁、四环素和克林霉素的耐药率分别为 75%、87.5%和 50%。ermF 和 nim 基因缺失，37.5%、12.5%、12.5%和 87.5%的菌株分别携带 cepA、cfiA、cfxA 和 tetQ 基因。10 株产 cfiA 基因阳性的拟杆菌和副拟杆菌菌株用 E-试验法检测亚胺培南和阿莫西林-克拉维酸，对亚胺培南的耐药率为 4.1%，对阿莫西林-克拉维酸的耐药率为 8.3%。这一特征在巴西尚属首次描述。
Zotero 插件