Toishi Yuko, Tsunoda Nobuo, Nagata Shun-Ichi, Kirisawa Rikio, Nagaoka Kentaro, Watanabe Gen, Yanagawa Yojiro, Katagiri Seiji, Taya Kazuyoshi
Shadai Stallion Station, Hokkaido 059-1432, Japan.
Laboratory of Veterinary Virology, Department of Pathobiology, School of Veterinary Medicine, Rakuno Gakuen University, Hokkaido 069-8501, Japan.
J Reprod Dev. 2018 Feb 27;64(1):41-47. doi: 10.1262/jrd.2017-099. Epub 2017 Nov 11.
Testosterone (T) concentration is a useful indicator of reproductive function in male animals. However, T concentration is not usually measured in veterinary clinics, partly due to the unavailability of reliable and rapid assays for animal samples. In this study, a rapid chemiluminescent enzyme immunoassay system (CLEIA system) that was developed for the measurement of T concentration in humans use was validated for stallion blood samples. First, serum T concentrations were measured using the CLEIA system and compared with those measured by a fluoroimmunoassay that has been validated for use in stallions. The serum T concentrations measured by the two methods were highly correlated (r = 0.9865, n = 56). Second, to validate the use of whole blood as assay samples, T concentrations in whole blood and in the serum were measured by the CLEIA system. T concentrations in both samples were highly correlated (r = 0.9665, n = 64). Finally, to evaluate the practical value of the CLEIA system in clinical settings, T concentrations were measured in three stallions with reproductive abnormalities after the administration of human chorionic gonadotropin (hCG). Two stallions with small or absent testes in the scrotum showed an increase in T production in response to hCG administration and one stallion with seminoma did not. In conclusion, the CLEIA system was found to be a rapid and reliable tool for measuring T concentrations in stallions and may improve reproductive management in clinical settings and in breeding studs.
睾酮（T）浓度是雄性动物生殖功能的一个有用指标。然而，兽医诊所通常不检测T浓度，部分原因是缺乏适用于动物样本的可靠且快速的检测方法。在本研究中，一种为测量人体T浓度而开发的快速化学发光酶免疫分析系统（CLEIA系统）在种马血液样本中得到了验证。首先，使用CLEIA系统测量血清T浓度，并与通过已在种马中验证的荧光免疫分析测量的浓度进行比较。两种方法测量的血清T浓度高度相关（r = 0.9865，n = 56）。其次，为了验证全血作为检测样本的用途，通过CLEIA系统测量全血和血清中的T浓度。两个样本中的T浓度高度相关（r = 0.9665，n = 64）。最后，为了评估CLEIA系统在临床环境中的实用价值，在给予人绒毛膜促性腺激素（hCG）后，测量了三匹患有生殖异常的种马的T浓度。两匹阴囊内睾丸小或无睾丸的种马对hCG给药显示T产生增加，而一匹患有精原细胞瘤的种马则没有。总之，发现CLEIA系统是测量种马T浓度的一种快速且可靠的工具，可能会改善临床环境和种马场的繁殖管理。
