Key Laboratory of Industrial Fermentation Microbiology, Ministry of Education, School of Biotechnology, Tianjin University of Science & Technology, Tianjin 300457, China.
Nanoscale. 2017 Nov 23;9(45):17699-17703. doi: 10.1039/c7nr07085c.
We report a novel assembly of polyethyleneimine (PEI)-coated FeO nanoparticles (NPs) with single-stranded DNA (ssDNA), and the fluorescence of the dye labeled in the DNA is remarkably quenched. In the presence of a target protein, the protein-DNA aptamer mutual interaction releases the ssDNA from this assembly and hence restores the fluorescence. This feature could be adopted to develop an aptasensor for protein detection. As a proof-of-concept, for the first time, we have used this proposed sensing strategy to detect thrombin selectively and sensitively. Furthermore, simultaneous multiple detection of thrombin and lysozyme in a complex protein mixture has been proven to be possible.
我们报告了一种新型的聚乙二胺（PEI）包覆的 FeO 纳米粒子（NPs）与单链 DNA（ssDNA）的组装，并且 DNA 中标记的染料的荧光显著猝灭。在存在靶蛋白的情况下，蛋白-DNA 适体相互作用将 ssDNA 从该组装体中释放出来，从而恢复荧光。这一特性可用于开发用于蛋白质检测的适体传感器。作为概念验证，我们首次使用这种传感策略来选择性和灵敏地检测凝血酶。此外，还证明了在复杂的蛋白质混合物中同时进行凝血酶和溶菌酶的多重检测是可能的。
