通过高效液相色谱法定量检测α-溶血素来鉴定葡萄球菌群体感应抑制剂

Identification of Staphylococcal Quorum Sensing Inhibitors by Quantification of õ-Hemolysin with High Performance Liquid Chromatography.

作者信息

Quave Cassandra L, Horswill Alexander R

机构信息

Department of Dermatology, Emory University School of Medicine, 615 Michael St., Whitehead 105L, Atlanta, GA, 30322, USA.

Center for the Study of Human Health, Emory University College of Arts and Sciences, 550 Asbury Circle, Candler Library 107, Atlanta, GA, 30322, USA.

出版信息

Methods Mol Biol. 2018;1673:363-370. doi: 10.1007/978-1-4939-7309-5_27.

DOI:10.1007/978-1-4939-7309-5_27

PMID:29130186

Abstract

Quorum sensing plays a major role in regulation of virulence factor production by staphylococci. Chemical inhibitors that block this process and prevent the production of exotoxins and exoenzymes could have medical utility for infection prophylaxis and therapy. Here, we describe a high performance liquid chromatography method amenable to medium throughput screening for staphylococcal quorum sensing inhibitors by quantification of õ-hemolysin, a direct protein output of this system.

摘要

群体感应在葡萄球菌毒力因子产生的调控中起主要作用。阻断这一过程并防止外毒素和外酶产生的化学抑制剂可能在感染预防和治疗中具有医学用途。在此，我们描述了一种高效液相色谱方法，该方法适用于通过定量该系统的直接蛋白质输出物——α-溶血素来对葡萄球菌群体感应抑制剂进行中等通量筛选。