a College of Sciences, Institute of Bioactive Compounds , Hue University , Hue , Vietnam.
b College of Food Industry , Da Nang , Vietnam.
Pharm Biol. 2017 Dec;55(1):2234-2239. doi: 10.1080/13880209.2017.1400077.
Eurycomanone is found in the Eurycoma longifolia Jack (Simaroubaceae) tree, exhibits significant antimalarial activity, improves spermatogenesis, suppresses expression of lung cancer cell tumour markers and regulates signalling pathways involved in proliferation, cell death and inflammation.
Establishment of cell suspension culture of E. longifolia to determine the eurycomanone accumulation during cultures.
Callus of E. longifolia was cultured in MS medium supplemented with 0.8% agar, 30/L sucrose, 1.25 mg/L NAA and 1 mg/L KIN for biomass production. Cell suspension culture was established by transferring friable calli to the same medium without agar. Eurycomanone content during cell culture was determined by HPLC with a C18 column, flow rate of 0.8 mL/min, run time of 17.5 min, detector wavelength of 254 nm. The stationary phase was silica gel and the mobile phase was acetonitric:HO. Roots of 5 year-old trees were used as the control.
The cells from 3 g of inoculum increased in biomass with a maximum value of 16 g fresh weight (0.7 g dry weight) at 14th day of culture. The cell growth then decreased from day 14 to day 20. Eurycomanone was produced during culture from the beginning to 20th day, its highest content (1.7 mg/g dry weight) also obtained at 14th day (the control is 2.1 mg/g dry weight).
Cell suspension culture of E. longifolia is a suitable procedure to produce eurycomanone. The yield of eurycomanone biosynthesis in 14 days-old cells are relatively high, approximately 0.8 times the control.
拉秧酮存在于长叶黄杨（Simaroubaceae）树中，具有显著的抗疟活性，可改善精子发生，抑制肺癌细胞肿瘤标志物的表达，并调节与增殖、细胞死亡和炎症相关的信号通路。
建立长叶黄杨细胞悬浮培养体系，以确定培养过程中拉秧酮的积累。
将长叶黄杨愈伤组织接种于添加 0.8%琼脂、30/L 蔗糖、1.25mg/L NAA 和 1mg/L KIN 的 MS 培养基中，以生产生物量。将易碎愈伤组织转移到不含琼脂的相同培养基中，建立细胞悬浮培养体系。采用 C18 柱、流速 0.8mL/min、运行时间 17.5min、检测波长 254nm 的 HPLC 法测定细胞培养过程中拉秧酮的含量。固定相为硅胶，流动相为乙腈：HO。5 年生树的根作为对照。
接种 3g 起始细胞的生物量在 14 天培养时增加到最大值 16g 鲜重（0.7g 干重）。细胞生长随后从第 14 天到第 20 天减少。从培养开始到第 20 天产生拉秧酮，其最高含量（1.7mg/g 干重）也在第 14 天获得（对照为 2.1mg/g 干重）。
长叶黄杨细胞悬浮培养是生产拉秧酮的合适方法。14 天龄细胞的拉秧酮生物合成产量相对较高，约为对照的 0.8 倍。
