School of Chemical Engineering and Technology, Tianjin University , No. 135 Yaguan Road, Haihe Education Park, Jinnan District, Tianjin 300350, P. R. China.
Laboratory of Organic NanoPhotonics and Key Laboratory of Bio-Inspired Materials and Interfacial Science, Technical Institute of Physics and Chemistry, Chinese Academy of Sciences , No. 29 Zhongguancun East Road, Beijing 100190, P. R. China.
ACS Appl Mater Interfaces. 2017 Dec 6;9(48):42247-42257. doi: 10.1021/acsami.7b14915. Epub 2017 Nov 22.
The microtechnology of controlling stimuli-responsive biomaterials at micrometer scale is crucial for biomedical applications. Here, we report bovine serum albumin (BSA)-based three-dimensional (3D) microstructures with tunable surface morphology and pH-responsive properties via two-photon polymerization microfabrication technology. The laser processing parameters, including laser power, scanning speed, and layer distance, are optimized for the fabrication of well-defined 3D BSA microstructures. The tunable morphology of BSA microstructures and a wide range of pH response corresponding to the swelling ratio of 1.08-2.71 have been achieved. The swelling behavior of the microstructures can be strongly influenced by the concentration of BSA precursor, which has been illustrated by a reasonable mechanism. A panda face-shaped BSA microrelief with reversible pH-responsive properties is fabricated and exhibits unique "facial expression" variations in pH cycle. We further design a mesh sieve-shaped microstructure as a functional device for promising microparticle separation. The pore sizes of microstructures can be tuned by changing the pH values. Therefore, such protein-based microstructures with controllable morphology and pH-responsive properties have potential applications especially in biomedicine and biosensors.
在微米尺度上控制刺激响应生物材料的微技术对于生物医学应用至关重要。在这里，我们通过双光子聚合微加工技术报道了基于牛血清白蛋白（BSA）的具有可调表面形态和 pH 响应特性的三维（3D）微结构。优化了激光加工参数，包括激光功率、扫描速度和层间距，以制造具有良好定义的 3D BSA 微结构。已经实现了 BSA 微结构的可调形态和与 1.08-2.71 的溶胀比相对应的宽 pH 响应范围。微结构的溶胀行为可以通过 BSA 前体的浓度强烈影响，这通过合理的机制得到了说明。制造了具有可逆 pH 响应特性的熊猫脸形状的 BSA 微浮雕，并在 pH 循环中表现出独特的“面部表情”变化。我们进一步设计了一种网格筛状微结构作为有前途的微粒子分离的功能器件。通过改变 pH 值可以调整微结构的孔径。因此，具有可控制形态和 pH 响应特性的这种基于蛋白质的微结构在生物医学和生物传感器中具有潜在的应用。
