Kocheshkova A A, Kroupin P Yu, Bazhenov M S, Karlov G I, Pochtovyy A A, Upelniek V P, Belov V I, Divashuk M G
Center for Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Moscow, Russia.
All-Russia Research Institute of Agricultural Biotechnology, Moscow, Russia.
PLoS One. 2017 Nov 13;12(11):e0188049. doi: 10.1371/journal.pone.0188049. eCollection 2017.
The germplasm collection of 87 wheat-wheatgrass hybrids developed in Tsitisin Main Botanical Garden (Russia, Moscow) was evaluated for resistance to pre-harvest sprouting (PHS) by spike sprouting (SS) and germination index (GI) assays as well as for spike and grain features. The PHS resistance variation and haplotype polymorphism of the wheatgrass ThVp-1 and wheat TaVp-1B genes orthologues of Vp-1 was revealed in the studied collection. Four haplotypes of ThVp-1 were revealed: ThVp-1a (41% of the entries), ThVp-1b (13%), ThVp-1c (29%), and ThVp-1d (15%). The association between the allelic state of ThVp-1 and PHS resistance in the wheat-wheatgrass hybrids was shown: haplotype ThVp-1d of the wheatgrass Vp-1 gene is significantly associated with reduced PHS in the wheat-wheatgrass hybrids (mean SS 0.33, mean GI 0.64). The resistant entries may be perspective as a source of PHS resistance in the development of commercial cultivars of perennial wheat.
对在季齐斯因主要植物园（俄罗斯莫斯科）培育的87个小麦-偃麦草杂交种的种质资源进行了评估，通过穗发芽（SS）和发芽指数（GI）测定法评估其对收获前发芽（PHS）的抗性，并评估了穗部和籽粒特征。在研究的种质资源中揭示了偃麦草ThVp-1和小麦TaVp-1B基因（Vp-1的直系同源基因）的PHS抗性变异和单倍型多态性。共揭示了ThVp-1的四种单倍型：ThVp-1a（占条目数的41%）、ThVp-1b（13%）、ThVp-1c（29%）和ThVp-1d（15%）。结果表明，小麦-偃麦草杂交种中ThVp-1的等位基因状态与PHS抗性之间存在关联：偃麦草Vp-1基因的单倍型ThVp-1d与小麦-偃麦草杂交种中PHS的降低显著相关（平均SS为0.33，平均GI为0.64）。这些抗性材料可能是多年生小麦商业品种开发中PHS抗性的潜在来源。
