Kroupin Pavel Yu, Kuznetsova Victoria M, Nikitina Ekaterina A, Martirosyan Yury Ts, Karlov Gennady I, Divashuk Mikhail G
Laboratory of Applied Genomics and Crop Breeding, All-Russia Research Institute of Agricultural Biotechnology, Timiryazevskaya str. 42, Moscow 127550, Russia All-Russia Research Institute of Agricultural Biotechnology Moscow Russia.
Center of Molecular Biotechnology, Russian State Agrarian University-Moscow Timiryazev Agricultural Academy, Timiryazevskaya str. 49, Moscow 127550, Russia Russian State Agrarian University-Moscow Timiryazev Agricultural Academ Moscow Russia.
Comp Cytogenet. 2019 Aug 13;13(3):231-243. doi: 10.3897/CompCytogen.v13i3.36112. eCollection 2019.
(Podpěra, 1902) Z.-W. Liu & R.-C.Wang, 1993 is an important polyploid wild perennial Triticeae species that is widely used as a source of valuable genes for wheat but its genomic constitution has long been debated. For its chromosome identification, only a limited set of FISH probes has been used. The development of new cytogenetic markers for chromosomes is of great importance both for cytogenetic characterization of wheat-wheatgrass hybrids and for fundamental comparative studies of phylogenetic relationships between species. Here, we report on the development of five cytogenetic markers for based on repetitive satellite DNA of which sequences were selected from the whole genome sequence of Cosson, 1849. Using real-time quantitative PCR we estimated the abundance of the found repeats: P720 and P427 had the highest abundance and P132, P332 and P170 had lower quantity in genome. Using fluorescence hybridization (FISH) we localized five repeats to different regions of the chromosomes of . Using reprobing multicolor FISH we colocalized the probes between each other. The distribution of these found repeats in the Triticeae genomes and its usability as cytogenetic markers for chromosomes of are discussed.
（波德佩拉，1902）Z.-W. 刘和R.-C. 王，1993年是一种重要的多倍体野生多年生小麦族物种，被广泛用作小麦宝贵基因的来源，但其基因组构成长期以来一直存在争议。对于其染色体鉴定，仅使用了有限的一组荧光原位杂交（FISH）探针。开发新的染色体细胞遗传学标记对于小麦 - 冰草属杂种的细胞遗传学特征以及物种间系统发育关系的基础比较研究都非常重要。在此，我们报告基于重复卫星DNA开发的五个用于[物种名称未明确]的细胞遗传学标记，其序列是从1849年科森的全基因组序列中选择的。使用实时定量PCR，我们估计了所发现重复序列的丰度：P720和P427丰度最高，而P132、P332和P170在[物种名称未明确]基因组中的数量较低。使用荧光原位杂交（FISH），我们将五个重复序列定位到[物种名称未明确]染色体的不同区域。使用再杂交多色FISH，我们将探针相互共定位。讨论了这些发现的重复序列在小麦族基因组中的分布及其作为[物种名称未明确]染色体细胞遗传学标记的可用性。
