Cui Mingming, Hu Ping, Wang Tao, Tao Jing, Zong Shixiang
Beijing Key Laboratory for Forest Pest Control, Beijing Forestry University, Beijing, P.R. China.
Mentougou Forestry Station, Beijing, China.
PLoS One. 2017 Nov 13;12(11):e0187105. doi: 10.1371/journal.pone.0187105. eCollection 2017.
Seabuckthorn carpenter moth, Eogystia hippophaecolus (Lepidoptera: Cossidae), is an important pest of sea buckthorn (Hippophae rhamnoides), which is a shrub that has significant ecological and economic value in China. E. hippophaecolus is highly cold tolerant, but limited studies have been conducted to elucidate the molecular mechanisms underlying its cold resistance. Here we sequenced the E. hippophaecolus transcriptome using RNA-Seq technology and performed de novo assembly from the short paired-end reads. We investigated the larval response to cold stress by comparing gene expression profiles between treatments. We obtained 118,034 unigenes, of which 22,161 were annotated with gene descriptions, conserved domains, gene ontology terms, and metabolic pathways. These resulted in 57 GO terms and 193 Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. By comparing transcriptome profiles for differential gene expression, we identified many differentially expressed proteins and genes, including heat shock proteins and cuticular proteins which have previously been reported to be involved in cold resistance of insects. This study provides a global transcriptome analysis and an assessment of differential gene expression in E. hippophaecolus under cold stress. We found seven differential expressed genes in common between developmental stages, which were verified with qPCR. Our findings facilitate future genomic studies aimed at improving our understanding of the molecular mechanisms underlying the response of insects to low temperatures.
沙棘木蠹蛾（Eogystia hippophaecolus，鳞翅目：木蠹蛾科）是沙棘（Hippophae rhamnoides）的一种重要害虫，沙棘在中国是一种具有重要生态和经济价值的灌木。沙棘木蠹蛾具有很强的耐寒性，但关于其抗寒分子机制的研究较少。在此，我们利用RNA-Seq技术对沙棘木蠹蛾转录组进行测序，并从短配对末端读段进行从头组装。通过比较不同处理之间的基因表达谱，我们研究了幼虫对冷胁迫的反应。我们获得了118,034个单基因，其中22,161个通过基因描述、保守结构域、基因本体论术语和代谢途径进行了注释。这些注释产生了57个基因本体论术语和193条京都基因与基因组百科全书（KEGG）途径。通过比较差异基因表达的转录组图谱，我们鉴定出许多差异表达的蛋白质和基因，包括先前报道参与昆虫抗寒的热休克蛋白和表皮蛋白。本研究提供了沙棘木蠹蛾的全局转录组分析以及对冷胁迫下差异基因表达的评估。我们发现发育阶段之间共有7个差异表达基因，并通过qPCR进行了验证。我们的研究结果有助于未来的基因组研究，旨在增进我们对昆虫对低温反应的分子机制的理解。
