Correia-Sá Luísa, Norberto Sónia, Delerue-Matos Cristina, Calhau Conceição, Domingues Valentina F
REQUIMTE/LAQV-GRAQ, Instituto Superior de Engenharia, Instituto Politécnico do Porto, Rua Dr. António Bernardino de Almeida, 431, 4200-072 Porto, Portugal; CINTESIS - Center for Research in Health Technologies and Information Systems, Porto, Portugal.
CINTESIS - Center for Research in Health Technologies and Information Systems, Porto, Portugal.
J Chromatogr B Analyt Technol Biomed Life Sci. 2018 Jan 1;1072:9-16. doi: 10.1016/j.jchromb.2017.10.060. Epub 2017 Nov 8.
Bisphenol A (BPA) is considered an endocrine disruptor and public concern over BPA exposure has been raised. Several studies have assessed human exposure to this plasticizer, confirming its ubiquitous presence and highlighting children as a public of special concern. A simple, efficient, cheap and green analytical procedure is reported within this paper. This paper reports, for the first time, the development of a modified Micro-QuEChERS (Quick, Easy, Cheap, Effective, Rugged and Safe) method coupled to gas chromatography-mass spectrometry (GC-MS) as a new strategy for the efficient extraction and determination of Bisphenol A in human urine samples. Several parameters that are known to influence extraction were optimized. Good linearity was achieved at the studied concentration range (1-50μg/L), with a correlation coefficient (R) of 0.998. The optimized method proved to be accurate (≥74% recovery), reproducible (<11% relative standard deviation) and sensitive for BPA determination (detection limit of 0.13μg/L and quantification limit of 0.43μg/L). The analytical procedure was applied to the analyses of 12 urine samples collected from children living in the North/Center region of Portugal. BPA was detected in all the analyzed samples in concentrations ranging from 1.5μg/L to 48.9μg/L. The proposed methodology is suitable for the determination of BPA in urine samples in the framework of biomonitoring studies and bioanalytical analyses, applying GC-MS detection.
双酚A（BPA）被认为是一种内分泌干扰物，公众对BPA暴露的担忧日益增加。多项研究评估了人类对这种增塑剂的暴露情况，证实其广泛存在，并强调儿童是特别值得关注的群体。本文报道了一种简单、高效、廉价且环保的分析方法。本文首次报道了一种改进的微快速、简便、廉价、有效、耐用和安全（Micro - QuEChERS）方法与气相色谱 - 质谱联用（GC - MS），作为一种高效提取和测定人尿液样本中双酚A的新策略。对几个已知会影响提取的参数进行了优化。在所研究的浓度范围（1 - 50μg/L）内实现了良好的线性，相关系数（R）为0.998。优化后的方法被证明对于双酚A的测定是准确的（回收率≥74%）、可重现的（相对标准偏差<11%）且灵敏的（检测限为0.13μg/L，定量限为0.43μg/L）。该分析方法应用于对从葡萄牙北部/中部地区儿童采集的12份尿液样本的分析。在所有分析样本中均检测到双酚A，浓度范围为1.5μg/L至48.9μg/L。所提出的方法适用于在生物监测研究和生物分析框架下，应用GC - MS检测测定尿液样本中的双酚A。
