Lu Xiao-Ying, Ouyang Yan-Qin, Zeng Wei-Ya, Lin Cui-Qing, Xiao Lu-Hua, Luo Gui-Hua, Zhan Ruo-Ting, Yan Ping
School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou 510006, China.
Key Laboratory of Chinese Medicinal Resources from Lingnan (Guangzhou University of Chinese Medicine), Ministry of Education, Guangzhou 510006, China.
J Anal Methods Chem. 2021 Dec 9;2021:8854025. doi: 10.1155/2021/8854025. eCollection 2021.
This study aimed to develop a method, followed by gas chromatography-mass spectrometry, for detecting 37 pesticides in () and investigating the decrease in the matrix-induced enhancement effect. The influence of QuEChERS extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared. extraction and matrix solid-phase dispersion (MSPD) on the recovery and matrix effect (ME) was compared to decrease the ME. The cleanup sorbents, volume and type of solvent, and treatment time were optimized. The accuracy (as recovery), precision (as relative standard deviation, RSD), linearity, limit of quantitation, and limit of detection were determined. The recoveries at the three levels using mixed standard solution ranged between 76% and 120% with RSD ≤15%, and 76% and 120% with RSD ≤11% for MSPD and QuEChERS extraction, respectively. The results suggested that the ME for 21 pesticides was in the range of 80%-120% after MSPD and 15% after QuEChERS extraction. QuEChERS extraction was simpler and faster than MSPD. This methodology was applied in the analysis of 27 samples; phorate was most frequently detected (63.0% of the sample).
本研究旨在开发一种继气相色谱 - 质谱联用之后用于检测（）中37种农药并研究基质诱导增强效应降低情况的方法。比较了QuEChERS萃取法和基质固相分散（MSPD）法对回收率和基质效应（ME）的影响。比较QuEChERS萃取法和基质固相分散（MSPD）法对回收率和基质效应（ME）的影响以降低基质效应。对净化吸附剂、溶剂体积和类型以及处理时间进行了优化。测定了准确度（以回收率表示）、精密度（以相对标准偏差，RSD表示）、线性、定量限和检测限。使用混合标准溶液时，三个加标水平下MSPD法和QuEChERS萃取法的回收率分别在76%至120%之间，RSD≤15%以及76%至120%之间，RSD≤11%。结果表明，21种农药经MSPD法处理后的基质效应在80% - 120%范围内，经QuEChERS萃取法处理后为15%。QuEChERS萃取法比MSPD法更简便、快速。该方法应用于27个样品的分析；甲拌磷检出频率最高（占样品的63.0%）。
