Lee Sun-Mi, Song Jun-Ho, Lee Kyo-Seok, Yoo Kyung-Hwa
Department of Physics, Yonsei University, Seoul 03722, Republic of Korea.
Nanomedical Graduate Program, Yonsei University, Seoul 03722, Republic of Korea.
Sensors (Basel). 2024 Dec 24;25(1):12. doi: 10.3390/s25010012.
The rapid and reliable detection of pathogenic bacteria remains a significant challenge in clinical microbiology. Consequently, the demand for simple and rapid techniques, such as antimicrobial peptide (AMP)-based sensors, has recently increased as an alternative to traditional methods. Melittin, a broad-spectrum AMP, rapidly associates with the cell membranes of various gram-positive and gram-negative bacteria. It also inhibits bacterial biofilm formation in blood culture media. In our study, bacterial growth was measured using electrical vertical-capacitance sensors with interdigitated electrodes functionalized with melittin, a widely studied AMP. The melittin-immobilized vertical-capacitance sensors demonstrated real-time detection of both standard and clinically isolated bacteria in media. Furthermore, these sensors successfully detected clinically isolated bacteria in blood culture media while inhibiting bacterial biofilm formation. Melittin-immobilized vertical-capacitance sensors provide a rapid and sensitive pathogen detection platform, with significant potential for improving patient care.
病原菌的快速可靠检测仍是临床微生物学面临的一项重大挑战。因此，作为传统方法的替代方案，对简单快速技术（如基于抗菌肽（AMP）的传感器）的需求近来有所增加。蜂毒肽是一种广谱抗菌肽，能迅速与各种革兰氏阳性菌和革兰氏阴性菌的细胞膜结合。它还能抑制血培养培养基中细菌生物膜的形成。在我们的研究中，使用带有经蜂毒肽（一种广泛研究的抗菌肽）功能化的叉指电极的垂直电容式电传感器来测量细菌生长。固定有蜂毒肽的垂直电容式传感器能够实时检测培养基中的标准菌株和临床分离菌株。此外，这些传感器在抑制细菌生物膜形成的同时，成功检测了血培养培养基中的临床分离菌株。固定有蜂毒肽的垂直电容式传感器提供了一个快速灵敏的病原体检测平台，在改善患者护理方面具有巨大潜力。
