Zhou Jie, Huang Jiabin, Huang Haoqiang, Zhao Cong, Zou Mengqiang, Liu Dejun, Weng Xiaoyu, Liu Liwei, Qu Junle, Liu Li, Liao Changrui, Wang Yiping
Key Laboratory of Optoelectronic Devices and Systems of Ministry of Education and Guangdong Province, College of Physics and Optoelectronic Engineering, Shenzhen University, Shenzhen 518060, China.
Shenzhen Key Laboratory of Photonic Devices and Sensing Systems for Internet of Things, Guangdong and Hong Kong Joint Research Centre for Optical Fiber Sensors, Shenzhen University, Shenzhen 518060, China.
Biomed Opt Express. 2023 Apr 3;14(5):1862-1873. doi: 10.1364/BOE.484015. eCollection 2023 May 1.
There is an urgent need for developing rapid and affordable antibiotic susceptibility testing (AST) technologies to inhibit the overuse of antibiotics. In this study, a novel microcantilever nanomechanical biosensor based on Fabry-Pérot interference demodulation was developed for AST. To construct the biosensor, a cantilever was integrated with the single mode fiber in order to form the Fabry-Pérot interferometer (FPI). After the attachment of bacteria on the cantilever, the fluctuations of cantilever caused by the bacterial movements were detected by monitoring the changes of resonance wavelength in the interference spectrum. We applied this methodology to and , showing the amplitude of cantilever's fluctuations was positively related on the quantity of bacteria immobilized on the cantilever and associated with the bacterial metabolism. The response of bacteria to antibiotics was dependent on the types of bacteria, the types and concentrations of antibiotics. Moreover, the minimum inhibitory and bactericidal concentrations for were obtained within 30 minutes, demonstrating the capacity of this method for rapid AST. Benefiting from the simplicity and portability of the optical fiber FPI-based nanomotion detection device, the developed nanomechanical biosensor in this study provides a promising technique for AST and a more rapid alternative for clinical laboratories.
迫切需要开发快速且经济实惠的抗生素敏感性测试（AST）技术，以抑制抗生素的过度使用。在本研究中，开发了一种基于法布里 - 珀罗干涉解调的新型微悬臂梁纳米机械生物传感器用于AST。为构建生物传感器，将悬臂梁与单模光纤集成以形成法布里 - 珀罗干涉仪（FPI）。细菌附着在悬臂梁上后，通过监测干涉光谱中共振波长的变化来检测由细菌运动引起的悬臂梁波动。我们将此方法应用于[具体细菌名称1]和[具体细菌名称2]，结果表明悬臂梁波动的幅度与固定在悬臂梁上的细菌数量呈正相关，且与细菌代谢有关。细菌对抗生素的反应取决于细菌类型、抗生素类型和浓度。此外，在30分钟内获得了[具体细菌名称]的最低抑菌浓度和最低杀菌浓度，证明了该方法用于快速AST的能力。受益于基于光纤FPI的纳米运动检测装置的简单性和便携性，本研究中开发的纳米机械生物传感器为AST提供了一种有前景的技术，是临床实验室更快速的替代方法。
