雌激素对大鼠子宫染色质蛋白修饰的影响。

Estrogen effects on modifications of chromatin proteins in the rat uterus.

作者信息

Guo J Z, Gorski J

机构信息

Department of Biochemistry, College of Agricultural and Life Sciences, University of Wisconsin-Madison 53706.

出版信息

J Steroid Biochem. 1989 Jan;32(1A):13-20. doi: 10.1016/0022-4731(89)90007-1.

DOI:10.1016/0022-4731(89)90007-1

PMID:2913395

Abstract

ADP-ribosylation and phosphorylation of chromatin proteins was studied in rat uterine nuclei isolated after estrogen treatment and then incubated with [adenylate-32P]NAD or [gamma-32P]ATP. Histone acetylation was studied in uteri from immature rats treated with estradiol by incubating the whole uterus in a medium containing [14C]acetic acid. Chromatin proteins were isolated from uterine nuclei and separated by electrophoresis on SDS polyacrylamide gels followed by autoradiography or fluorography. Chromatin proteins H1, H2B, H3, HMG 14 and HMG 17 were almost exclusively ADP-ribosylated. Uterine histones H1, H3, H4, HMG 14 and HMG 17 were phosphorylated. There was a general increase in [32P]ADP-ribose uptake in chromatin proteins after estrogen stimulation, whereas [32P]phosphate incorporation into chromatin proteins showed a biphasic pattern. The [14C]acetate activity associated with all histone proteins increased gradually after estrogen treatment.

摘要

研究了雌激素处理后分离的大鼠子宫细胞核中染色质蛋白的ADP-核糖基化和磷酸化情况，随后将其与[腺苷酸-32P]NAD或[γ-32P]ATP一起孵育。通过将未成熟大鼠的整个子宫在含有[14C]乙酸的培养基中孵育，研究了用雌二醇处理的未成熟大鼠子宫中的组蛋白乙酰化情况。从子宫细胞核中分离染色质蛋白，并在SDS聚丙烯酰胺凝胶上进行电泳分离，然后进行放射自显影或荧光自显影。染色质蛋白H1、H2B、H3、HMG 14和HMG 17几乎完全被ADP-核糖基化。子宫组蛋白H1、H3、H4、HMG 14和HMG 17被磷酸化。雌激素刺激后，染色质蛋白中[32P]ADP-核糖的摄取普遍增加，而[32P]磷酸盐掺入染色质蛋白呈现双相模式。雌激素处理后，与所有组蛋白相关的[14C]乙酸活性逐渐增加。